Male C57BL/6J mice (Jackson Laboratories) were housed on a 12-hour light-dark cycle and were allowed free access to water and standard laboratory chow or high fat diet (Research Diet D12450Bi, D12492i) for 22-24 weeks. Adenoviruses were purified and diluted in saline solution, and 2E11 viral particles were delivered into the animals by a single tail-vein injection on day zero. Animals were fed ad libitum and were sacrificed at day 5 after adenovirus injection.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from pulverized liver tissues using Qiagen RNeasy 96 Universal Tissue 8000 Kit.
Label
biotin
Label protocol
Enzo Biochem
Hybridization protocol
Midi_euk2v3
Scan protocol
Scan protocol: GeneChips were scanned using an Affymetrix GeneChip Scanner 3000
Description
Jnk1 Adv-shRNA treated liver from C57BL/6J DIO mice.
