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Sample GSM18503 Query DataSets for GSM18503
Status Public on Mar 13, 2004
Title 72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 2
Sample type RNA
 
Channel 1
Source name 72 h serum-deprived T98G cells with 30 min PDGF treatment
Organism Homo sapiens
Extracted molecule total RNA
 
Channel 2
Source name 72hr serum starved T98G cells (untreated)
Organism Homo sapiens
Extracted molecule total RNA
 
 
Description T98G human glioblastoma cells were grown in Minimal Essential Medium (Invitrogen) supplemented with fetal calf serum (10%). For growth factor/inhibitor treatments, cells were incubated in serum-free medium for 72 h, and either left unstimulated, or stimulated for 30 min with human PDGF-BB (50 ng/ml) (Sigma). U0126 (Cell Signaling Technology) and LY294002 (BioMol) were added 60 min prior to PDGF addition.
 
Submission date Mar 11, 2004
Last update date May 27, 2005
Contact name Geoffrey M Cooper
Organization name Boston University
Department Biology
Lab
Street address 5 Cummington St
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL875
Series (1)
GSE1128 PDGF-induced genes regulated by the PI3K and MEK/ERK signaling pathways

Data table header descriptions
ID_REF
VALUE Average log2 ratio (from dye swap)
DS1_CH1_MEDIAN Dye Swap Array 1: Foreground 635 Channel Median (Treated)
DS1_CH1_SD Dye Swap Array 1: Foreground 635 Channel Standard Deviation (Treated)
DS1_CH1_BKD_MEDIAN Dye Swap Array 1: Background 635 Channel Median (Treated)
DS1_CH1_BKD_SD Dye Swap Array 1: Background 635 Channel Standard Deviation (Treated)
DS1_CH2_MEDIAN Dye Swap Array 1: Foreground 532 Channel Median (Untreated)
DS1_CH2_SD Dye Swap Array 1: Foreground 532 Channel Standard Deviation (Untreated)
DS1_CH2_BKD_MEDIAN Dye Swap Array 1: Background 532 Channel Median (Untreated)
DS1_CH2_BKD_SD Dye Swap Array 1: Background 532 Channel Standard Deviation (Untreated)
DS2_CH1_MEDIAN Dye Swap Array 2: Foreground 635 Channel Median (Untreated)
DS2_CH1_SD Dye Swap Array 2: Foreground 635 Channel Standard Deviation (Untreated)
DS2_CH1_BKD_MEDIAN Dye Swap Array 2: Background 635 Channel Median (Untreated)
DS2_CH1_BKD_SD Dye Swap Array 2: Background 635 Channel Standard Deviation (Untreated)
DS2_CH2_MEDIAN Dye Swap Array 2: Foreground 532 Channel Median (Treated)
DS2_CH2_SD Dye Swap Array 2: foreground 532 Channel Standard Deviation (Treated)
DS2_CH2_BKD_MEDIAN Dye Swap Array 2: Background 532 Channel Median (Treated)
DS2_CH2_BKD_SD Dye Swap Array 2: Background 532 Channel Standard Deviation (Treated)
QUALITY 0: ignore, 1: good spot

Data table
ID_REF VALUE DS1_CH1_MEDIAN DS1_CH1_SD DS1_CH1_BKD_MEDIAN DS1_CH1_BKD_SD DS1_CH2_MEDIAN DS1_CH2_SD DS1_CH2_BKD_MEDIAN DS1_CH2_BKD_SD DS2_CH1_MEDIAN DS2_CH1_SD DS2_CH1_BKD_MEDIAN DS2_CH1_BKD_SD DS2_CH2_MEDIAN DS2_CH2_SD DS2_CH2_BKD_MEDIAN DS2_CH2_BKD_SD QUALITY
1 NULL 13581 3180 500 146 65535 7025 433 1829 15277 5962 212 112 65535 12440 301 2359 1
2 NULL 399 1744 487 200 413 407 412 173 633 287 206 230 411 93 297 107 0
3 0.127 18420 5369 475 153 17133 4846 417 148 18143 4967 218 139 20215 5247 304 100 1
4 NULL 573 153 467 277 641 143 414 187 388 110 218 86 563 138 305 76 1
5 NULL 440 258 473 796 442 379 419 790 256 188 214 117 361 101 301 74 0
6 -0.083 1456 319 480 357 1352 298 435 298 1624 432 218 129 1485 387 307 72 1
7 NULL 420 178 475 181 492 137 437 130 385 123 217 123 448 99 307 72 1
8 -0.107 750 226 464 140 947 235 416 109 602 161 213 98 925 244 302 76 1
9 NULL 435 142 460 834 472 113 422 710 308 227 210 147 417 123 302 74 0
10 0.21 6085 2329 469 229 4924 2340 414 186 6101 1881 211 149 6630 1387 305 72 1
11 -0.089 784 262 481 1076 865 261 418 1001 603 183 212 123 819 216 309 76 1
12 0.023 1521 337 485 1152 1409 281 430 1032 1480 311 222 102 1545 384 317 78 1
13 0.175 3508 720 479 215 4195 976 426 158 2997 945 219 181 4711 1147 307 157 1
14 NULL 389 358 470 283 483 372 420 235 436 174 218 108 462 84 307 87 0
15 -0.065 1536 445 468 241 2142 556 416 239 1362 332 210 100 2009 425 308 89 1
16 NULL 404 162 465 136 397 111 415 141 258 137 207 83 335 68 300 69 0
17 0.036 664 148 463 167 640 145 413 121 516 200 207 79 670 143 303 67 1
18 NULL 384 223 468 133 454 183 410 113 602 228 223 83 498 72 306 68 0
19 NULL 365 122 460 134 408 102 414 115 309 133 214 95 396 79 305 142 0
20 0.234 1368 447 464 458 1143 369 414 333 1166 328 216 100 1365 291 305 142 1

Total number of rows: 18564

Table truncated, full table size 1394 Kbytes.




Supplementary file Size Download File type/resource
GSM18503.TIF.gz 115.9 Mb (ftp)(http) TIFF

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