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Sample GSM18507

Query DataSets for GSM18507

Status

Public on Mar 13, 2004

Title

72 h serum-deprived T98G cells with 1 h LY294002 and 30 min PDGF treatments, replicate 1

Sample type

RNA

Channel 1

Source name

72 h serum-deprived T98G cells with 1 h LY294002 and 30 min PDGF treatments

Organism

Extracted molecule

total RNA

Channel 2

Source name

72hr serum starved T98G cells (untreated)

Organism

Extracted molecule

total RNA

Description

T98G human glioblastoma cells were grown in Minimal Essential Medium (Invitrogen) supplemented with fetal calf serum (10%). For growth factor/inhibitor treatments, cells were incubated in serum-free medium for 72 h, and either left unstimulated, or stimulated for 30 min with human PDGF-BB (50 ng/ml) (Sigma). U0126 (Cell Signaling Technology) and LY294002 (BioMol) were added 60 min prior to PDGF addition.

Submission date

Mar 11, 2004

Last update date

May 27, 2005

Contact name

Geoffrey M Cooper

Organization name

Boston University

Department

Biology

Lab

Street address

5 Cummington St

City

Boston

State/province

MA

ZIP/Postal code

02215

Country

USA

Platform ID

Series (1)

PDGF-induced genes regulated by the PI3K and MEK/ERK signaling pathways

Data table header descriptions
ID_REF
VALUE	Average log2 ratio (from dye swap)
DS1_CH1_MEDIAN	Dye Swap Array 1: Foreground 635 Channel Median (Treated)
DS1_CH1_SD	Dye Swap Array 1: Foreground 635 Channel Standard Deviation (Treated)
DS1_CH1_BKD_MEDIAN	Dye Swap Array 1: Background 635 Channel Median (Treated)
DS1_CH1_BKD_SD	Dye Swap Array 1: Background 635 Channel Standard Deviation (Treated)
DS1_CH2_MEDIAN	Dye Swap Array 1: Foreground 532 Channel Median (Untreated)
DS1_CH2_SD	Dye Swap Array 1: Foreground 532 Channel Standard Deviation (Untreated)
DS1_CH2_BKD_MEDIAN	Dye Swap Array 1: Background 532 Channel Median (Untreated)
DS1_CH2_BKD_SD	Dye Swap Array 1: Background 532 Channel Standard Deviation (Untreated)
DS2_CH1_MEDIAN	Dye Swap Array 2: Foreground 635 Channel Median (Untreated)
DS2_CH1_SD	Dye Swap Array 2: Foreground 635 Channel Standard Deviation (Untreated)
DS2_CH1_BKD_MEDIAN	Dye Swap Array 2: Background 635 Channel Median (Untreated)
DS2_CH1_BKD_SD	Dye Swap Array 2: Background 635 Channel Standard Deviation (Untreated)
DS2_CH2_MEDIAN	Dye Swap Array 2: Foreground 532 Channel Median (Treated)
DS2_CH2_SD	Dye Swap Array 2: foreground 532 Channel Standard Deviation (Treated)
DS2_CH2_BKD_MEDIAN	Dye Swap Array 2: Background 532 Channel Median (Treated)
DS2_CH2_BKD_SD	Dye Swap Array 2: Background 532 Channel Standard Deviation (Treated)
QUALITY	0: ignore, 1: good spot

Data table
ID_REF	VALUE	DS1_CH1_MEDIAN	DS1_CH1_SD	DS1_CH1_BKD_MEDIAN	DS1_CH1_BKD_SD	DS1_CH2_MEDIAN	DS1_CH2_SD	DS1_CH2_BKD_MEDIAN	DS1_CH2_BKD_SD	DS2_CH1_MEDIAN	DS2_CH1_SD	DS2_CH1_BKD_MEDIAN	DS2_CH1_BKD_SD	DS2_CH2_MEDIAN	DS2_CH2_SD	DS2_CH2_BKD_MEDIAN	DS2_CH2_BKD_SD	QUALITY
1	NULL	4810	2038	135	67	30791	16966	229	74	6374	1435	140	74	34606	11228	181	57	1
2	NULL	231	105	132	72	266	86	230	74	175	97	137	96	173	64	165	55	0
3	-0.272	15113	2401	139	85	12975	3118	231	70	11510	1933	142	122	6804	1828	169	64	1
4	NULL	304	106	134	70	362	91	227	70	208	88	138	66	222	63	168	53	1
5	NULL	173	78	127	62	262	77	224	70	151	65	132	78	171	61	162	78	0
6	-0.242	891	220	129	63	792	228	224	68	587	174	127	63	406	105	161	51	1
7	NULL	247	96	124	62	290	80	228	76	182	86	124	68	181	51	158	54	0
8	-0.09	370	118	125	77	400	149	224	81	272	96	130	68	248	67	158	55	1
9	NULL	188	89	123	62	265	89	223	68	164	80	126	65	181	57	159	50	0
10	-0.542	1605	439	124	61	1615	512	223	70	1438	372	127	67	742	230	161	52	1
11	-0.448	363	128	123	62	477	133	217	70	321	109	132	66	276	76	166	61	1
12	-0.2	716	203	126	63	668	179	218	70	611	175	130	65	443	115	165	52	1
13	NULL	117	56	121	64	213	67	210	66	130	62	125	64	158	49	163	52	0
14	-0.065	241	85	120	63	297	79	213	65	208	97	126	123	215	83	163	94	1
15	-0.353	784	221	122	69	925	212	214	76	831	203	129	503	624	169	162	651	1
16	NULL	129	67	119	68	234	77	209	76	121	64	124	347	176	50	161	555	0
17	-0.254	291	105	117	63	347	82	206	66	296	101	124	64	260	69	162	51	1
18	0.183	246	238	115	59	318	250	212	65	203	79	132	66	242	58	168	52	1
19	NULL	176	95	116	63	253	74	209	68	185	80	134	124	195	55	169	151	0
20	-0.143	423	138	114	62	425	125	205	67	432	145	134	123	344	123	170	164	1

Total number of rows: 18564

Table truncated, full table size 1334 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM18507.TIF.gz	115.0 Mb	(ftp)(http)	TIFF

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