|
| Status |
Public on Aug 18, 2015 |
| Title |
rat at C3, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Rats which were supplied with tap water for 2 weeks.
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: kidney
|
| Treatment protocol |
experimental (T) group for the gene and protein expression studies and control (C) group. Rats in T group were provided free access to drinking water containing 1% (vol/vol) ethylene glycol and 0.5% ammonium chloride for a period of 2 weeks in order to induce hyperoxaluria, while the rats in C group were supplied with tap water for 2 weeks.
|
| Growth protocol |
all rats were acclimated at 23℃ on a 12 h light/dark cycle before initiation of experiments in the SPF level animal house.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
About 1ug total RNA were polyA tailed and labled with FlashTag™ Biotin HSR Labeling Kit(Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
The labled RNA were hybridized for 16 hr at 60 rpm at 48C on Affymetrix miRNA4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from rats which were supplied with tap water for 2 weeks.
|
| Data processing |
Raw data files were analyzed by using Affymetrix Expression Console with the default miRNA array settings. Raw data were processed and normalized on basis of organism rat using RMA-DABG algorithm.
|
| |
|
| Submission date |
Aug 18, 2015 |
| Last update date |
Aug 18, 2015 |
| Contact name |
Jiang Hongyang |
| E-mail(s) |
jiang.hongyang@163.com
|
| Organization name |
Tongji Hospital, Huazhong University of Science and Technology
|
| Street address |
No. 1095 Jie Fang Avenue
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430000 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE72135 |
Expression data from hyperoxaluric rats |
|
