GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM185954 Query DataSets for GSM185954
Status Public on Jun 30, 2008
Title pcdh12-deficient mice rep1
Sample type RNA
Source name wild-type placenta embryonic day 12.5
Organism Mus musculus
Characteristics Gender:male
Growth protocol mice were mated and the day on which a vaginal plug was found was designated 0.5.
Extracted molecule total RNA
Extraction protocol Pregnant females were killed by cervical dislocation and conceptuses were dissected in PBS. Total RNA were extracted from E12.5 placentas using RNeasy kit (Qiagen). RNA concentration and integrity were tested with an Agilent 2100 Bioanalyzer (Agilent Technologies Inc.).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol 12,5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450, protocol EukGE_WS2v5_450
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 
Description PH_M1_430_2
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date May 01, 2007
Last update date Aug 28, 2018
Contact name Philippe HUBER
Organization name Inserm-CEA
Department iRTSV
Lab Lab U882 – Vascular Physiopathology
Street address 17, Rue des martyrs
ZIP/Postal code 38054
Country France
Platform ID GPL1261
Series (1)
GSE7676 Changes in gene expression following Protocadherin 12 knockout
Reanalyzed by GSE119085

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
1415670_at 574.4 P 0.000244
1415671_at 1090.8 P 0.000732
1415672_at 1044.5 P 0.000244
1415673_at 173.5 P 0.000732
1415674_a_at 355.6 P 0.000244
1415675_at 309.7 P 0.000244
1415676_a_at 1312.7 P 0.000244
1415677_at 154 P 0.000244
1415678_at 376.3 P 0.000244
1415679_at 963.8 P 0.000244
1415680_at 426.1 P 0.000244
1415681_at 528.5 P 0.000244
1415682_at 118.3 P 0.001221
1415683_at 971 P 0.000244
1415684_at 122.1 P 0.000244
1415685_at 177 P 0.000244
1415686_at 326.4 P 0.000244
1415687_a_at 6152.2 P 0.000244
1415688_at 392.9 P 0.000244
1415689_s_at 102.5 P 0.000732

Total number of rows: 45101

Table truncated, full table size 1188 Kbytes.

Supplementary file Size Download File type/resource
GSM185954.CEL.gz 5.4 Mb (ftp)(http) CEL
GSM185954.EXP.gz 364 b (ftp)(http) EXP
Raw data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap