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Sample GSM185958 Query DataSets for GSM185958
Status Public on Jun 30, 2008
Title pcdh12-deficient mice rep5
Sample type RNA
 
Source name wild-type placenta embryonic day 12.5
Organism Mus musculus
Characteristics Gender:male
Growth protocol mice were mated and the day on which a vaginal plug was found was designated 0.5.
Extracted molecule total RNA
Extraction protocol Pregnant females were killed by cervical dislocation and conceptuses were dissected in PBS. Total RNA were extracted from E12.5 placentas using RNeasy kit (Qiagen). RNA concentration and integrity were tested with an Agilent 2100 Bioanalyzer (Agilent Technologies Inc.).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol 12,5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450, protocol EukGE_WS2v5_450
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 
Description PH_M5_430_2
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 01, 2007
Last update date Aug 28, 2018
Contact name Philippe HUBER
E-mail(s) phuber@cea.fr
Organization name Inserm-CEA
Department iRTSV
Lab Lab U882 – Vascular Physiopathology
Street address 17, Rue des martyrs
City GRENOBLE
ZIP/Postal code 38054
Country France
 
Platform ID GPL1261
Series (1)
GSE7676 Changes in gene expression following Protocadherin 12 knockout
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 647.8 P 0.000244
1415671_at 971.7 P 0.000732
1415672_at 1006.5 P 0.000244
1415673_at 174.4 P 0.001221
1415674_a_at 391.5 P 0.000244
1415675_at 339.1 P 0.000244
1415676_a_at 1255.2 P 0.000244
1415677_at 172 P 0.000244
1415678_at 355.2 P 0.000244
1415679_at 842.5 P 0.000244
1415680_at 425.9 P 0.000244
1415681_at 499.4 P 0.000244
1415682_at 190.9 P 0.000244
1415683_at 1005.8 P 0.000244
1415684_at 121.5 P 0.000244
1415685_at 159.8 P 0.000244
1415686_at 321.6 P 0.000244
1415687_a_at 5112 P 0.000244
1415688_at 364 P 0.000244
1415689_s_at 112.3 P 0.001953

Total number of rows: 45101

Table truncated, full table size 1188 Kbytes.




Supplementary file Size Download File type/resource
GSM185958.CEL.gz 5.5 Mb (ftp)(http) CEL
GSM185958.EXP.gz 364 b (ftp)(http) EXP
Raw data provided as supplementary file

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