|
| Status |
Public on Jun 30, 2008 |
| Title |
Wild-type mice rep2 |
| Sample type |
RNA |
| |
|
| Source name |
pcdh12-deficient placenta embryonic day 12.5
|
| Organism |
Mus musculus |
| Characteristics |
Gender:male
|
| Growth protocol |
mice were mated and the day on which a vaginal plug was found was designated 0.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pregnant females were killed by cervical dislocation and conceptuses were dissected in PBS. Total RNA were extracted from E12.5 placentas using RNeasy kit (Qiagen). RNA concentration and integrity were tested with an Agilent 2100 Bioanalyzer (Agilent Technologies Inc.).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
12,5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450, protocol EukGE_WS2v5_450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
PH_WT4_430_2
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 01, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Philippe HUBER |
| E-mail(s) |
phuber@cea.fr
|
| Organization name |
Inserm-CEA
|
| Department |
iRTSV
|
| Lab |
Lab U882 – Vascular Physiopathology
|
| Street address |
17, Rue des martyrs
|
| City |
GRENOBLE |
| ZIP/Postal code |
38054 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7676 |
Changes in gene expression following Protocadherin 12 knockout |
|
| Relations |
| Reanalyzed by |
GSE119085 |
