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Sample GSM185960 Query DataSets for GSM185960
Status Public on Jun 30, 2008
Title Wild-type mice rep2
Sample type RNA
 
Source name pcdh12-deficient placenta embryonic day 12.5
Organism Mus musculus
Characteristics Gender:male
Growth protocol mice were mated and the day on which a vaginal plug was found was designated 0.5.
Extracted molecule total RNA
Extraction protocol Pregnant females were killed by cervical dislocation and conceptuses were dissected in PBS. Total RNA were extracted from E12.5 placentas using RNeasy kit (Qiagen). RNA concentration and integrity were tested with an Agilent 2100 Bioanalyzer (Agilent Technologies Inc.).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol 12,5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450, protocol EukGE_WS2v5_450
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 
Description PH_WT4_430_2
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 01, 2007
Last update date Aug 28, 2018
Contact name Philippe HUBER
E-mail(s) phuber@cea.fr
Organization name Inserm-CEA
Department iRTSV
Lab Lab U882 – Vascular Physiopathology
Street address 17, Rue des martyrs
City GRENOBLE
ZIP/Postal code 38054
Country France
 
Platform ID GPL1261
Series (1)
GSE7676 Changes in gene expression following Protocadherin 12 knockout
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 440.7 P 0.000244
1415671_at 982 P 0.000244
1415672_at 1299.4 P 0.000244
1415673_at 257.8 P 0.000732
1415674_a_at 420.7 P 0.000244
1415675_at 488.9 P 0.000244
1415676_a_at 1558.4 P 0.000244
1415677_at 137 P 0.000732
1415678_at 425.8 P 0.000244
1415679_at 952.5 P 0.000244
1415680_at 297.6 P 0.000244
1415681_at 520.3 P 0.000244
1415682_at 105.4 P 0.000732
1415683_at 920.4 P 0.000244
1415684_at 163.1 P 0.000244
1415685_at 189.1 P 0.000244
1415686_at 274 P 0.000244
1415687_a_at 4464.3 P 0.000244
1415688_at 450 P 0.000244
1415689_s_at 119.5 P 0.000732

Total number of rows: 45101

Table truncated, full table size 1189 Kbytes.




Supplementary file Size Download File type/resource
GSM185960.CEL.gz 5.6 Mb (ftp)(http) CEL
GSM185960.EXP.gz 363 b (ftp)(http) EXP
Raw data provided as supplementary file

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