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Sample GSM1862914 Query DataSets for GSM1862914
Status Public on Jun 29, 2016
Title Baseline_rep2
Sample type RNA
 
Source name Muscle biopsy before intervention derived from subject 2
Organism Homo sapiens
Characteristics insulin sensitivity: non-responder
tissue: muscle
Sex: female
age: 62
Treatment protocol As described in the paper. Briefly, before and after the training period, all participants underwent lactate diagnostics and maximal bicycle spiroergometry to determine the individual anaerobic threshold (IAT) and maximal oxygen uptake (VO2max). The training program lasted 8 weeks and consisted of three supervised exercise sessions per week. Each training session consisted of 30 min each bicycle ergometer exercise and 30 min treadmill exercise. The training intensity was individually set at 80% of VO2max, based on the performance test and controlled by heart rate.
Extracted molecule total RNA
Extraction protocol miRNeasy Mini kit (Qiagen) including DNase digestion
Label biotin
Label protocol Ovation Pico WTA System V2 and Encore Biotin Module (Nugen)
 
Hybridization protocol According to the Affymetrix expression protocol (HWS kit) including minor changes as suggested by the Encore Biotin protocol
Scan protocol According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
Description Gene expression data from muscle biopsy before intervention derived from subject 2
Data processing RMA probeset summary with standard settings of the Affymetrix Expression Console software (build 1.4.0.38) including median polish and Sketch-Quantile normalisation
 
Submission date Aug 27, 2015
Last update date Mar 01, 2019
Contact name Johannes Beckers
E-mail(s) beckers@helmholtz-muenchen.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL17586
Series (1)
GSE72462 TGFβ contributes to impaired exercise response by suppression of mitochondrial key regulators in skeletal muscle

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2)

Data table
ID_REF VALUE
TC01000001.hg.1 4.427
TC01000002.hg.1 3.559
TC01000003.hg.1 2.185
TC01000004.hg.1 3.552
TC01000005.hg.1 6.388
TC01000006.hg.1 9.989
TC01000007.hg.1 10.014
TC01000008.hg.1 4.836
TC01000009.hg.1 2.207
TC01000010.hg.1 2.307
TC01000011.hg.1 4.384
TC01000012.hg.1 2.920
TC01000013.hg.1 5.279
TC01000014.hg.1 3.884
TC01000015.hg.1 4.467
TC01000016.hg.1 3.852
TC01000017.hg.1 4.337
TC01000018.hg.1 6.288
TC01000019.hg.1 4.315
TC01000020.hg.1 4.606

Total number of rows: 70523

Table truncated, full table size 1526 Kbytes.




Supplementary file Size Download File type/resource
GSM1862914_BL_02.CEL.gz 22.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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