|
| Status |
Public on Jun 29, 2016 |
| Title |
Baseline_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Muscle biopsy before intervention derived from subject 5
|
| Organism |
Homo sapiens |
| Characteristics |
insulin sensitivity: non-responder
tissue: muscle
Sex: male
age: 40
|
| Treatment protocol |
As described in the paper. Briefly, before and after the training period, all participants underwent lactate diagnostics and maximal bicycle spiroergometry to determine the individual anaerobic threshold (IAT) and maximal oxygen uptake (VO2max). The training program lasted 8 weeks and consisted of three supervised exercise sessions per week. Each training session consisted of 30 min each bicycle ergometer exercise and 30 min treadmill exercise. The training intensity was individually set at 80% of VO2max, based on the performance test and controlled by heart rate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini kit (Qiagen) including DNase digestion
|
| Label |
biotin
|
| Label protocol |
Ovation Pico WTA System V2 and Encore Biotin Module (Nugen)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit) including minor changes as suggested by the Encore Biotin protocol
|
| Scan protocol |
According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
|
| Description |
Gene expression data from muscle biopsy before intervention derived from subject 5
|
| Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (build 1.4.0.38) including median polish and Sketch-Quantile normalisation
|
| |
|
| Submission date |
Aug 27, 2015 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE72462 |
TGFβ contributes to impaired exercise response by suppression of mitochondrial key regulators in skeletal muscle |
|
