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Sample GSM1862922 Query DataSets for GSM1862922
Status Public on Jun 29, 2016
Title Baseline_rep10
Sample type RNA
 
Source name Muscle biopsy before intervention derived from subject 10
Organism Homo sapiens
Characteristics insulin sensitivity: low-responder
tissue: muscle
Sex: female
age: 43
Treatment protocol As described in the paper. Briefly, before and after the training period, all participants underwent lactate diagnostics and maximal bicycle spiroergometry to determine the individual anaerobic threshold (IAT) and maximal oxygen uptake (VO2max). The training program lasted 8 weeks and consisted of three supervised exercise sessions per week. Each training session consisted of 30 min each bicycle ergometer exercise and 30 min treadmill exercise. The training intensity was individually set at 80% of VO2max, based on the performance test and controlled by heart rate.
Extracted molecule total RNA
Extraction protocol miRNeasy Mini kit (Qiagen) including DNase digestion
Label biotin
Label protocol Ovation Pico WTA System V2 and Encore Biotin Module (Nugen)
 
Hybridization protocol According to the Affymetrix expression protocol (HWS kit) including minor changes as suggested by the Encore Biotin protocol
Scan protocol According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
Description Gene expression data from muscle biopsy before intervention derived from subject 10
Data processing RMA probeset summary with standard settings of the Affymetrix Expression Console software (build 1.4.0.38) including median polish and Sketch-Quantile normalisation
 
Submission date Aug 27, 2015
Last update date Mar 01, 2019
Contact name Johannes Beckers
E-mail(s) beckers@helmholtz-muenchen.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL17586
Series (1)
GSE72462 TGFβ contributes to impaired exercise response by suppression of mitochondrial key regulators in skeletal muscle

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2)

Data table
ID_REF VALUE
TC01000001.hg.1 4.171
TC01000002.hg.1 3.569
TC01000003.hg.1 2.335
TC01000004.hg.1 3.534
TC01000005.hg.1 6.258
TC01000006.hg.1 9.433
TC01000007.hg.1 9.128
TC01000008.hg.1 4.948
TC01000009.hg.1 2.241
TC01000010.hg.1 2.713
TC01000011.hg.1 4.423
TC01000012.hg.1 3.269
TC01000013.hg.1 9.214
TC01000014.hg.1 3.951
TC01000015.hg.1 4.296
TC01000016.hg.1 3.578
TC01000017.hg.1 4.448
TC01000018.hg.1 6.400
TC01000019.hg.1 4.467
TC01000020.hg.1 4.720

Total number of rows: 70523

Table truncated, full table size 1526 Kbytes.




Supplementary file Size Download File type/resource
GSM1862922_BL_10.CEL.gz 22.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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