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Sample GSM1862924 Query DataSets for GSM1862924
Status Public on Jun 29, 2016
Title Baseline_rep12
Sample type RNA
Source name Muscle biopsy before intervention derived from subject 12
Organism Homo sapiens
Characteristics insulin sensitivity: high-responder
tissue: muscle
Sex: female
age: 45
Treatment protocol As described in the paper. Briefly, before and after the training period, all participants underwent lactate diagnostics and maximal bicycle spiroergometry to determine the individual anaerobic threshold (IAT) and maximal oxygen uptake (VO2max). The training program lasted 8 weeks and consisted of three supervised exercise sessions per week. Each training session consisted of 30 min each bicycle ergometer exercise and 30 min treadmill exercise. The training intensity was individually set at 80% of VO2max, based on the performance test and controlled by heart rate.
Extracted molecule total RNA
Extraction protocol miRNeasy Mini kit (Qiagen) including DNase digestion
Label biotin
Label protocol Ovation Pico WTA System V2 and Encore Biotin Module (Nugen)
Hybridization protocol According to the Affymetrix expression protocol (HWS kit) including minor changes as suggested by the Encore Biotin protocol
Scan protocol According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
Description Gene expression data from muscle biopsy before intervention derived from subject 12
Data processing RMA probeset summary with standard settings of the Affymetrix Expression Console software (build including median polish and Sketch-Quantile normalisation
Submission date Aug 27, 2015
Last update date Mar 01, 2019
Contact name Johannes Beckers
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
Platform ID GPL17586
Series (1)
GSE72462 TGFβ contributes to impaired exercise response by suppression of mitochondrial key regulators in skeletal muscle

Data table header descriptions
VALUE RMA signal intensity (log2)

Data table
TC01000001.hg.1 3.917
TC01000002.hg.1 3.673
TC01000003.hg.1 2.460
TC01000004.hg.1 3.334
TC01000005.hg.1 6.301
TC01000006.hg.1 9.492
TC01000007.hg.1 9.506
TC01000008.hg.1 4.863
TC01000009.hg.1 2.346
TC01000010.hg.1 2.444
TC01000011.hg.1 4.480
TC01000012.hg.1 2.982
TC01000013.hg.1 9.036
TC01000014.hg.1 3.875
TC01000015.hg.1 4.239
TC01000016.hg.1 3.894
TC01000017.hg.1 4.358
TC01000018.hg.1 6.248
TC01000019.hg.1 4.299
TC01000020.hg.1 4.521

Total number of rows: 70523

Table truncated, full table size 1526 Kbytes.

Supplementary file Size Download File type/resource
GSM1862924_BL_12.CEL.gz 22.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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