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Sample GSM1862943 Query DataSets for GSM1862943
Status Public on Jun 29, 2016
Title Post_intervention_13
Sample type RNA
 
Source name Muscle biopsy after an eight weeks endurance training intervention derived from subject 13
Organism Homo sapiens
Characteristics insulin sensitivity: low-responder
tissue: muscle
Sex: male
age: 54
Treatment protocol As described in the paper. Briefly, before and after the training period, all participants underwent lactate diagnostics and maximal bicycle spiroergometry to determine the individual anaerobic threshold (IAT) and maximal oxygen uptake (VO2max). The training program lasted 8 weeks and consisted of three supervised exercise sessions per week. Each training session consisted of 30 min each bicycle ergometer exercise and 30 min treadmill exercise. The training intensity was individually set at 80% of VO2max, based on the performance test and controlled by heart rate.
Extracted molecule total RNA
Extraction protocol miRNeasy Mini kit (Qiagen) including DNase digestion
Label biotin
Label protocol Ovation Pico WTA System V2 and Encore Biotin Module (Nugen)
 
Hybridization protocol According to the Affymetrix expression protocol (HWS kit) including minor changes as suggested by the Encore Biotin protocol
Scan protocol According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G)
Description Gene expression data from muscle biopsy after an eight weeks endurance training intervention derived from subject 13
Data processing RMA probeset summary with standard settings of the Affymetrix Expression Console software (build 1.4.0.38) including median polish and Sketch-Quantile normalisation
 
Submission date Aug 27, 2015
Last update date Mar 01, 2019
Contact name Johannes Beckers
E-mail(s) beckers@helmholtz-muenchen.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL17586
Series (1)
GSE72462 TGFβ contributes to impaired exercise response by suppression of mitochondrial key regulators in skeletal muscle

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2)

Data table
ID_REF VALUE
TC01000001.hg.1 3.625
TC01000002.hg.1 3.586
TC01000003.hg.1 2.528
TC01000004.hg.1 3.388
TC01000005.hg.1 6.321
TC01000006.hg.1 9.769
TC01000007.hg.1 9.678
TC01000008.hg.1 4.816
TC01000009.hg.1 2.399
TC01000010.hg.1 2.308
TC01000011.hg.1 4.342
TC01000012.hg.1 3.273
TC01000013.hg.1 8.370
TC01000014.hg.1 3.644
TC01000015.hg.1 4.393
TC01000016.hg.1 3.817
TC01000017.hg.1 4.009
TC01000018.hg.1 6.243
TC01000019.hg.1 4.319
TC01000020.hg.1 4.602

Total number of rows: 70523

Table truncated, full table size 1526 Kbytes.




Supplementary file Size Download File type/resource
GSM1862943_PI_13.CEL.gz 22.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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