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Sample GSM1863292 Query DataSets for GSM1863292
Status Public on Apr 15, 2016
Title Activated 1
Sample type RNA
 
Source name Primary myoblasts
Organism Mus musculus
Characteristics strain: C57bl6N
expression: active form of beta-catenin
Treatment protocol Differentiating myocytes were either treated with 4-OH tamoxifen (diluted in ethanol) to induce recombination of beta-catenin gene or with ethanol alone for control cells.
Growth protocol Cells were grown in Ham F10 media with 20%FBS and 2ng/ml bFGF for 5 passage. Differentiation was induced by serum removal and 24hours of culture in DMEM with 2% HS.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
 
Hybridization protocol Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
Data processing the data were RMA normalized and analysed with Partek Genomic Suit
 
Submission date Aug 28, 2015
Last update date Apr 15, 2016
Contact name Fabien Le Grand
E-mail(s) fabien.le-grand@inserm.fr
Phone 0033144412436
Organization name Institut Cochin
Department DRC
Street address 24 rue du Fg Saint Jacques
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL18802
Series (1)
GSE72496 Expression data from primary myoblasts with active Beta-catenin

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
11287_at 3.43407601
11298_at 3.643941585
11302_at 4.511116313
11303_at 3.175038246
11304_at 3.225044178
11305_at 5.619253189
11306_at 6.582694603
11307_at 4.5483069
11308_at 7.242319748
11350_at 5.350003927
11352_at 6.747278696
11354_at 3.466705273
11363_at 8.95425555
11364_at 7.143936301
11370_at 5.700622903
11409_at 5.674889057
11416_at 7.874166621
11418_at 3.779885219
11419_at 3.791090316
11421_at 3.357176545

Total number of rows: 24479

Table truncated, full table size 515 Kbytes.




Supplementary file Size Download File type/resource
GSM1863292_Activated_1_23_3_MoGene-2_0-st_.CEL.gz 7.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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