|
| Status |
Public on Apr 15, 2016 |
| Title |
Activated 3 |
| Sample type |
RNA |
| |
|
| Source name |
Primary myoblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57bl6N
expression: active form of beta-catenin
|
| Treatment protocol |
Differentiating myocytes were either treated with 4-OH tamoxifen (diluted in ethanol) to induce recombination of beta-catenin gene or with ethanol alone for control cells.
|
| Growth protocol |
Cells were grown in Ham F10 media with 20%FBS and 2ng/ml bFGF for 5 passage. Differentiation was induced by serum removal and 24hours of culture in DMEM with 2% HS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Data processing |
the data were RMA normalized and analysed with Partek Genomic Suit
|
| |
|
| Submission date |
Aug 28, 2015 |
| Last update date |
Apr 15, 2016 |
| Contact name |
Fabien Le Grand |
| E-mail(s) |
fabien.le-grand@inserm.fr
|
| Phone |
0033144412436
|
| Organization name |
Institut Cochin
|
| Department |
DRC
|
| Street address |
24 rue du Fg Saint Jacques
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL18802 |
| Series (1) |
| GSE72496 |
Expression data from primary myoblasts with active Beta-catenin |
|
