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Sample GSM1869179 Query DataSets for GSM1869179
Status Public on Dec 31, 2016
Title DS#16
Sample type RNA
 
Source name tumor tissue
Organism Homo sapiens
Characteristics tissue type: medullary thyroid carcinoma
Extracted molecule total RNA
Extraction protocol Total RNA was obtained following the manufacturer’s instructions using the TriReagent kit (MRC, Cincinnati, OH, USA).
Label Hy3
Label protocol RNA was labeled with Hy3 fluorescent dye according to the manufacturer’s instructions using miRCURY LNATM microRNA Array kit (Exiqon).
 
Hybridization protocol Samples were hybridized over 16 h at 56°C onto miRNA microarray (Exiqon, v.11.0-hsa, mmu & rho) according to the manufacturer’s instructions using miRCURY LNATM microRNA Array kit (Exiqon).
Scan protocol The slides were scanned in an Agilent microarray scanner (Agilent Technologies, Palo Alto, CA, USA).
Description tumoral sample 16
Data processing Fluorescence intensities on scanned one-color images were quantified using Feature Extraction v9.5 software (Agilent Technologies) using the modified Exiqon protocol. Background correction was carried out, and raw data were normalized by quantiles.
Raw data were normalized by quantiles and probes belonging to the same microRNA gene were merged by median.
 
Submission date Sep 04, 2015
Last update date Dec 31, 2016
Contact name Mercedes Robledo
E-mail(s) mrobledo@cnio.es
Organization name Spanish National Cancer Research Centre (CNIO)
Department Human Cancer Genetics Program
Lab Hereditary Endocrine Cancer Group
Street address C/ Melchor Fernández Almagro, 3
City Madrid
State/province Madrid
ZIP/Postal code 28029
Country Spain
 
Platform ID GPL17107
Series (2)
GSE72728 Integration of miRNA, mRNA and methylation profiling reveals genes aberrantly regulated associated to RETM918T mutation in Medullary Thyroid Carcinoma [miRNA component]
GSE72730 Integration of miRNA, mRNA and methylation profiling reveals genes aberrantly regulated associated to RETM918T mutation in Medullary Thyroid Carcinoma

Supplementary file Size Download File type/resource
GSM1869179_13994210.txt.gz 797.0 Kb (ftp)(http) TXT
Processed data are available on Series record

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