|
Status |
Public on Dec 31, 2016 |
Title |
DS#16 |
Sample type |
RNA |
|
|
Source name |
tumor tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue type: medullary thyroid carcinoma
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was obtained following the manufacturer’s instructions using the TriReagent kit (MRC, Cincinnati, OH, USA).
|
Label |
Hy3
|
Label protocol |
RNA was labeled with Hy3 fluorescent dye according to the manufacturer’s instructions using miRCURY LNATM microRNA Array kit (Exiqon).
|
|
|
Hybridization protocol |
Samples were hybridized over 16 h at 56°C onto miRNA microarray (Exiqon, v.11.0-hsa, mmu & rho) according to the manufacturer’s instructions using miRCURY LNATM microRNA Array kit (Exiqon).
|
Scan protocol |
The slides were scanned in an Agilent microarray scanner (Agilent Technologies, Palo Alto, CA, USA).
|
Description |
tumoral sample 16
|
Data processing |
Fluorescence intensities on scanned one-color images were quantified using Feature Extraction v9.5 software (Agilent Technologies) using the modified Exiqon protocol. Background correction was carried out, and raw data were normalized by quantiles. Raw data were normalized by quantiles and probes belonging to the same microRNA gene were merged by median.
|
|
|
Submission date |
Sep 04, 2015 |
Last update date |
Dec 31, 2016 |
Contact name |
Mercedes Robledo |
E-mail(s) |
mrobledo@cnio.es
|
Organization name |
Spanish National Cancer Research Centre (CNIO)
|
Department |
Human Cancer Genetics Program
|
Lab |
Hereditary Endocrine Cancer Group
|
Street address |
C/ Melchor Fernández Almagro, 3
|
City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28029 |
Country |
Spain |
|
|
Platform ID |
GPL17107 |
Series (2) |
GSE72728 |
Integration of miRNA, mRNA and methylation profiling reveals genes aberrantly regulated associated to RETM918T mutation in Medullary Thyroid Carcinoma [miRNA component] |
GSE72730 |
Integration of miRNA, mRNA and methylation profiling reveals genes aberrantly regulated associated to RETM918T mutation in Medullary Thyroid Carcinoma |
|