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Sample GSM187352 Query DataSets for GSM187352
Status Public on Oct 21, 2008
Title frontal cortex control, biological rep2
Sample type RNA
 
Source name Mus musculus frontal cortex, saline i.p.
Organism Mus musculus
Characteristics Strain: C57BL/6J
Gender: male
Age: 14 weeks
Tissue: frontal cortex
Treatment: saline i.p.
Treatment protocol Animals were randomly assigned to either the MPTP (n = 3) or saline (n = 3) groups. The MPTP treatment consisted of 30 mg/kg MPTP, i.p., calculated as the free base (MPTP-HCl, Sigma) administered once daily for 5 days. At four days after the last MPTP injection, the animals were briefly anaesthetized and immediately sacrificed by decapitation.
Extracted molecule total RNA
Extraction protocol RNeasy Lipid Tissue Mini Kit (Qiagen Inc., Chatsworth, USA) was used for extraction of total RNA and performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, cRNA were hybridized on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
Description Gene expression data from frontal cortex, saline i.p.
Data processing The data were analyzed with Robust Microarray Analysis (RMA)
 
Submission date May 04, 2007
Last update date Aug 28, 2018
Contact name Daniel M Sforza
E-mail(s) dsforza@gmu.edu
Organization name George Mason University
Department Computational and Data Sciences
Street address 4400 University Drive
City Fairfax
State/province VA
ZIP/Postal code 22030
Country USA
 
Platform ID GPL1261
Series (1)
GSE7707 Gene expression changes in multiple brain regions of a mouse MPTP model of Parkinson's disease
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
1415670_at 9.567979015
1415671_at 10.90813957
1415672_at 9.811004259
1415673_at 7.93442332
1415674_a_at 8.938758185
1415675_at 8.472528876
1415676_a_at 10.49855389
1415677_at 8.681663864
1415678_at 10.29042769
1415679_at 9.505830512
1415680_at 7.850636728
1415681_at 8.883347978
1415682_at 8.008434817
1415683_at 9.917958507
1415684_at 6.657018457
1415685_at 7.586290132
1415686_at 9.710218565
1415687_a_at 12.24643263
1415688_at 9.718349859
1415689_s_at 5.774384018

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM187352.CEL.gz 3.6 Mb (ftp)(http) CEL

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