|
| Status |
Public on Oct 21, 2008 |
| Title |
midbrain control, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mus musculus midbrain, saline i.p.
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6J
Gender: male
Age: 14 weeks
Tissue: midbrain
Treatment: saline i.p.
|
| Treatment protocol |
Animals were randomly assigned to either the MPTP (n = 3) or saline (n = 3) groups. The MPTP treatment consisted of 30 mg/kg MPTP, i.p., calculated as the free base (MPTP-HCl, Sigma) administered once daily for 5 days. At four days after the last MPTP injection, the animals were briefly anaesthetized and immediately sacrificed by decapitation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Lipid Tissue Mini Kit (Qiagen Inc., Chatsworth, USA) was used for extraction of total RNA and performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
|
| Description |
Gene expression data from midbrain, saline i.p.
|
| Data processing |
The data were analyzed with Robust Microarray Analysis (RMA)
|
| |
|
| Submission date |
May 04, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Daniel M Sforza |
| E-mail(s) |
dsforza@gmu.edu
|
| Organization name |
George Mason University
|
| Department |
Computational and Data Sciences
|
| Street address |
4400 University Drive
|
| City |
Fairfax |
| State/province |
VA |
| ZIP/Postal code |
22030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7707 |
Gene expression changes in multiple brain regions of a mouse MPTP model of Parkinson's disease |
|
| Relations |
| Reanalyzed by |
GSE119085 |
