|
Status |
Public on Oct 21, 2008 |
Title |
striatum mptp, biological rep1 |
Sample type |
RNA |
|
|
Source name |
Mus musculus striatum, MPTP i.p.
|
Organism |
Mus musculus |
Characteristics |
Strain: C57BL/6J Gender: male Age: 14 weeks Tissue: striatum Treatment: MPTP i.p.
|
Treatment protocol |
Animals were randomly assigned to either the MPTP (n = 3) or saline (n = 3) groups. The MPTP treatment consisted of 30 mg/kg MPTP, i.p., calculated as the free base (MPTP-HCl, Sigma) administered once daily for 5 days. At four days after the last MPTP injection, the animals were briefly anaesthetized and immediately sacrificed by decapitation.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Lipid Tissue Mini Kit (Qiagen Inc., Chatsworth, USA) was used for extraction of total RNA and performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
|
Description |
Gene expression data from striatum, MPTP i.p.
|
Data processing |
The data were analyzed with Robust Microarray Analysis (RMA)
|
|
|
Submission date |
May 04, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Daniel M Sforza |
E-mail(s) |
dsforza@gmu.edu
|
Organization name |
George Mason University
|
Department |
Computational and Data Sciences
|
Street address |
4400 University Drive
|
City |
Fairfax |
State/province |
VA |
ZIP/Postal code |
22030 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7707 |
Gene expression changes in multiple brain regions of a mouse MPTP model of Parkinson's disease |
|
Relations |
Reanalyzed by |
GSE119085 |