|
| Status |
Public on Jun 07, 2017 |
| Title |
replicate 3 KO run |
| Sample type |
RNA |
| |
|
| Source name |
KO run
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: KO
protocol: exercise
tissue: heart
|
| Treatment protocol |
animals were sacrified at day 14 of treatment protocol and heart were collected in liquid nitrogen
|
| Growth protocol |
Animals were running on a treadmill for 2x 5 days with age of 8 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
gene expression data from HDAC4 knockout mouse after 2 weeks forced running exercise
KO_pos_1151
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of mouse430_2-type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Sep 10, 2015 |
| Last update date |
Jun 07, 2017 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18376 |
| Series (1) |
| GSE72900 |
Role of Histone Deacetylase 4 on cardiac expression after exercise |
|
