|
| Status |
Public on Feb 14, 2008 |
| Title |
Stomach - Wildtype - 1 |
| Sample type |
RNA |
| |
|
| Source name |
Stomach tissue
|
| Organism |
Mus musculus |
| Characteristics |
STRAIN : C57BL/6J
GENDER : MALE
TISSUE : STOMACH
DIET : CHOW
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were food deprived beginning at 1300 (during the light period) and euthanized 4 h later with an overdose of isoflurane inhalation followed by decapitation. The stomach was quickly removed, rinsed with cold PBS, frozen in liquid nitrogen, and stored at –80 deg. C. RNA was isolated using TRIzol extraction protocol. Quality and quantity was assesed by Agilent bioanalyzer (Agilent Technologies, Palo Alto, CA)
|
| Label |
DIG-UTP
|
| Label protocol |
1 microgram of total RNA was used to transcribe DIG labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT kit v2.0
|
| |
|
| Hybridization protocol |
Fifteen microgram of DIG labeled fragmented cRNA was used for microarray hybridization and performed according to the Applied Biosystems protocols
|
| Scan protocol |
Chemiluminescence detection, imaging, auto girding, and image analysis was done according to the Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v1.0.3
|
| Description |
Two strains were used – B6.CAST-17 congenic and WT for this study. Each strain was represented by two pools for a total of four samples from each strain. Thus, two biological replicates were analyzed for each strain.
|
| Data processing |
Signal intensities across microarrays were normalized using quantile-quantile normalization method (www.bioconductor.org)
|
| |
|
| Submission date |
May 07, 2007 |
| Last update date |
Jan 13, 2012 |
| Contact name |
Brenda Richards |
| E-mail(s) |
richarbk@pbrc.edu
|
| Organization name |
Pennington Biomedical Research Center
|
| Department |
Basic Science
|
| Lab |
Genetics of Eating Behavior
|
| Street address |
6400 Perkins Rd.
|
| City |
Baton Rouge |
| State/province |
LA |
| ZIP/Postal code |
70808 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE7756 |
Genetic variation in Glp1r expression influences the rate of gastric emptying |
|
