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Sample GSM187994 Query DataSets for GSM187994
Status Public on Jun 14, 2007
Title SWR/J SAL, Rep3
Sample type RNA
 
Source name SWR/J Saline treated, biological replicate 3
Organism Mus musculus
Characteristics Striatum of 8-10 week old SWR/J mice
Treatment protocol Morphine (morphine hydrochloride; Polfa, Kutno, Poland) was administered subcutaneously (s.c.). Experimental groups (control, acute and chronic morphine) consisted of 9 animals from each strain. To obtain the most reliable comparison, control and acute morphine groups received injections of saline for four days in the same time schedule as the chronic group received morphine. On the fifth day, acute treated animals were injected with a single dose of morphine (20 mg/kg) and killed by decapitation after 4 h. Chronically treated animals were injected with increasing doses of morphine for five days. Mice received morphine thrice daily (09:00 h, 13:00 h and 17:00 h) for four days using a dosing schedule of 10, 20, 40 and 40 mg/kg of morphine on days 1, 2, 3 and 4, respectively. On the last day, a final morphine dose of 40 mg/kg was administered and 4 h after the last injection, the animals were sacrificed. Mice in control groups were killed 4 h after the last injection of saline. The dose scheme and time schedule were used in order to maximize strains differences in response to morphine.
Growth protocol Adult male (8 to 10 weeks of age) 129P3/J (000690), DBA/2J (000671), C57BL/6J (000664) and SWR/J (000689) mice (The Jackson Laboratory, Bar Harbor, Maine, USA) were housed 6 per cage, under a 12 h dark/light cycle, with ad libitum access to food and water. Animals weighing from 20 to 30 g were used throughout the experiments. The animal protocols used in the study were approved by the local Bioethics Commission working at the Institute of Pharmacology, Polish Academy of Sciences (Krakow, Poland).
Extracted molecule total RNA
Extraction protocol Trizol/RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following labeling, samples were hybridized to the GeneChip Test3 array (Affymetrix, Santa Clara, CA) for quality control. Fragmented cRNA (15 μg) was used for hybridization to the GeneChip Mouse Genome 430 2.0 arrays (Affymetrix, Santa Clara, CA). Arrays were washed and stained with streptavidin-phycoerythrin (Merck, Darmstadt, Germany) in Fluidic Station 400 (Affymetrix, Santa Clara, CA) according to standard protocol of the manufacturer.
Scan protocol The arrays were scanned using the GeneChip Scanner 3000 (Affymetrix, Santa Clara, CA).
Description SWR/J Saline treated, striatum, biological replicate 3
Data processing Data was normalized by dChip 2006 (MBEI)
 
Submission date May 08, 2007
Last update date Aug 28, 2018
Contact name Marcin Piechota
E-mail(s) marpiech@if-pan.krakow.pl
Phone (+4812) 6623328
Organization name Institute of Pharmacology PAS
Department Molecular Neuropharmacology
Street address Smetna 12
City Krakow
ZIP/Postal code 31-343
Country Poland
 
Platform ID GPL1261
Series (1)
GSE7762 Morphine effects on striatal transcriptome in four inbred mouse strains
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MBEI PM/MM signal intensity
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 334.304 P
AFFX-BioB-M_at 346.174 P
AFFX-BioB-3_at 319.566 P
AFFX-BioC-5_at 766.318 P
AFFX-BioC-3_at 698.992 P
AFFX-BioDn-5_at 1412.79 P
AFFX-BioDn-3_at 2242.95 P
AFFX-CreX-5_at 4812.55 P
AFFX-CreX-3_at 4357.17 P
AFFX-DapX-5_at 2421.39 P
AFFX-DapX-M_at 3514.61 P
AFFX-DapX-3_at 3715.9 P
AFFX-LysX-5_at 7449.82 P
AFFX-LysX-M_at 7900.8 P
AFFX-LysX-3_at 8009.3 P
AFFX-PheX-5_at 5033.24 P
AFFX-PheX-M_at 5420.37 P
AFFX-PheX-3_at 5908.39 P
AFFX-ThrX-5_at 6194.99 P
AFFX-ThrX-M_at 6818.77 P

Total number of rows: 45101

Table truncated, full table size 938 Kbytes.




Supplementary file Size Download File type/resource
GSM187994.CEL.gz 3.4 Mb (ftp)(http) CEL

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