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Sample GSM188072 Query DataSets for GSM188072
Status Public on Jun 21, 2007
Title Adult Hind Gut biological rep1
Sample type RNA
 
Source name Adult Hindgut
Organism Drosophila melanogaster
Characteristics tissue: Canton-S, 60 female and 60 male flies, 7 days old
Treatment protocol Flies were anaesthetised briefly by chilling on ice, then immediately dissected.
Growth protocol Wild-type Drosophila melanogaster (Canton S strain) were raised on standard medium on a 12:12 h L:D cycle, at 23C, and at 55% r.h. To facilitate the collection of accurately staged adults, a laying population of around 12 males and 12 females were transferred to fresh vials daily. When adults subsequently emerged, they were transferred to fresh vials on the day of emergence, and used 7 days later. Where larvae were used, these were third instar.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNeasy Micro kit (Invitrogen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA (or according to the affy IVT protocol) were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip scanner 3000 7G.
Description The hindgut, from just behind the insertion of the Malpighian tubules, and including the rectum, severing at the anus
amplification: 1-round
Data processing The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 09, 2007
Last update date Aug 28, 2018
Contact name Jing Wang or Venkat Chintapalli
E-mail(s) jw128h@udcf.gla.ac.uk, vrc1h@udcf.gla.ac.uk
Phone 0141 3306212
Fax 3304878
Organization name University of Glasgow
Department Molecular Genetics
Lab Dow/Davis lab
Street address Dumbarton Road
City Glasgow
State/province Scotland
ZIP/Postal code G11 6NU
Country United Kingdom
 
Platform ID GPL1322
Series (1)
GSE7763 Using FlyAtlas to identify better Drosophila models of human disease
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE GCOS1.4 signal intensity (MAS 5)
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 98.726 P 0.000195116
AFFX-BioB-M_at 130.651 P 0.000126798
AFFX-BioB-3_at 77.4066 P 0.000509415
AFFX-BioC-5_at 205.164 P 6.02111e-05
AFFX-BioC-3_at 203.62 P 4.42873e-05
AFFX-BioDn-5_at 543.753 P 4.42873e-05
AFFX-BioDn-3_at 1208.68 P 6.02111e-05
AFFX-CreX-5_at 3326.03 P 4.42873e-05
AFFX-CreX-3_at 3896.54 P 4.42873e-05
AFFX-DapX-5_at 2.88662 M 0.054198
AFFX-DapX-M_at 1.03185 A 0.824693
AFFX-DapX-3_at 5.98125 A 0.58862
AFFX-LysX-5_at 2.30381 A 0.440646
AFFX-LysX-M_at 1.7204 A 0.794268
AFFX-LysX-3_at 5.09438 A 0.185131
AFFX-PheX-5_at 0.271558 A 0.883887
AFFX-PheX-M_at 0.386984 A 0.995002
AFFX-PheX-3_at 3.62445 A 0.574038
AFFX-ThrX-5_at 0.790654 A 0.772364
AFFX-ThrX-M_at 3.31832 A 0.574044

Total number of rows: 18952

Table truncated, full table size 580 Kbytes.




Supplementary file Size Download File type/resource
GSM188072.CEL.gz 2.0 Mb (ftp)(http) CEL
GSM188072.CHP.gz 108.3 Kb (ftp)(http) CHP
GSM188072.xml.gz 4.6 Kb (ftp)(http) XML
Processed data included within Sample table
Processed data provided as supplementary file

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