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Sample GSM188097 Query DataSets for GSM188097
Status Public on Jun 21, 2007
Title Adult Head biological rep2
Sample type RNA
 
Source name Adult Head
Organism Drosophila melanogaster
Characteristics tissue: Canton-S, 50 female and 50 male flies, 7 days old
Treatment protocol Flies were anaesthetised briefly by chilling on ice, then immediately dissected.
Growth protocol Wild-type Drosophila melanogaster (Canton S strain) were raised on standard medium on a 12:12 h L:D cycle, at 23C, and at 55% r.h. To facilitate the collection of accurately staged adults, a laying population of around 12 males and 12 females were transferred to fresh vials daily. When adults subsequently emerged, they were transferred to fresh vials on the day of emergence, and used 7 days later. Where larvae were used, these were third instar.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNeasy Micro kit (Invitrogen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA (or according to the affy IVT protocol) were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip scanner 3000 7G.
Description The head capsule, including the eyes and brain.
amplification: 1-round
Data processing The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 09, 2007
Last update date Aug 28, 2018
Contact name Jing Wang or Venkat Chintapalli
E-mail(s) jw128h@udcf.gla.ac.uk, vrc1h@udcf.gla.ac.uk
Phone 0141 3306212
Fax 3304878
Organization name University of Glasgow
Department Molecular Genetics
Lab Dow/Davis lab
Street address Dumbarton Road
City Glasgow
State/province Scotland
ZIP/Postal code G11 6NU
Country United Kingdom
 
Platform ID GPL1322
Series (1)
GSE7763 Using FlyAtlas to identify better Drosophila models of human disease
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE GCOS1.4 signal intensity (MAS 5)
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 100.7 P 5.16732e-05
AFFX-BioB-M_at 135.799 P 7.00668e-05
AFFX-BioB-3_at 80.4314 P 0.000224668
AFFX-BioC-5_at 240.133 P 0.000126798
AFFX-BioC-3_at 248.709 P 5.16732e-05
AFFX-BioDn-5_at 592.709 P 4.42873e-05
AFFX-BioDn-3_at 1232.11 P 4.42873e-05
AFFX-CreX-5_at 3456.05 P 5.16732e-05
AFFX-CreX-3_at 4265.38 P 4.42873e-05
AFFX-DapX-5_at 0.210239 A 0.966347
AFFX-DapX-M_at 0.629229 A 0.883887
AFFX-DapX-3_at 1.50977 A 0.794268
AFFX-LysX-5_at 0.616282 A 0.804734
AFFX-LysX-M_at 1.34141 A 0.824672
AFFX-LysX-3_at 0.658188 A 0.659339
AFFX-PheX-5_at 0.60717 A 0.631562
AFFX-PheX-M_at 0.438506 A 0.978098
AFFX-PheX-3_at 4.05288 A 0.60308
AFFX-ThrX-5_at 2.77824 A 0.368438
AFFX-ThrX-M_at 3.55961 A 0.5

Total number of rows: 18952

Table truncated, full table size 582 Kbytes.




Supplementary file Size Download File type/resource
GSM188097.CEL.gz 1.9 Mb (ftp)(http) CEL
GSM188097.CHP.gz 108.2 Kb (ftp)(http) CHP
GSM188097.xml.gz 7.6 Kb (ftp)(http) XML
Processed data included within Sample table
Processed data provided as supplementary file

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