|
Status |
Public on Jul 15, 2007 |
Title |
C57BL6J mice infected peritoneal lavage day 3pi, biological repeat 5 |
Sample type |
RNA |
|
|
Source name |
C57BL6J mice infected peritoneal lavage day 3pi, biological repeat 5
|
Organism |
Mus musculus |
Characteristics |
C57BL6J 7 wk old Male
|
Treatment protocol |
Chlamydia psittaci (10,000 IFU) was suspended in 200 microL of PBS and injected intraperitoneally
|
Growth protocol |
6 week old male C57BL6J and DBA2J mice were purchased from Jackson Laboratories and maintained in our animal care facility for 1 week prior to use
|
Extracted molecule |
total RNA |
Extraction protocol |
Peritoneal lavage was collected sterily by injection and collection of PBS into the peritoneal cavity. Extracted lavage was centrifuged and supernatent was discarded. Cell pellets were treated with Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Transcription profile of peritoneal lavage cells 3 days post infection with Chlamydia psittaci
|
Data processing |
The data were analyzed with Robust multiple array average using default analysis settings and global scaling as normalization method. RMA data normalized to 8 with SD of 2 units.
|
|
|
Submission date |
May 09, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Lorne A Rose |
E-mail(s) |
lrose4@utmem.edu
|
Phone |
901-448-4459
|
Organization name |
University of Tennessee
|
Department |
Molecular Sciences
|
Street address |
854 Madision Ave
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38163 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7767 |
Differential transcription of ip Chlamydia infected C57BL6J and DBA2J mice |
|
Relations |
Reanalyzed by |
GSE119085 |