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Sample GSM188246 Query DataSets for GSM188246
Status Public on May 18, 2007
Title GFP- CD25- CD4+ CD8- thymocytes from Foxp3(gfp/gfp) mice
Sample type RNA
 
Source name thymocytes
Organism Mus musculus
Characteristics Strain: C57BL/6 backcross 4-6 from 129/Sv
Gender: Female Age: 6-8 weeks
Treatment protocol Cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy
Label biotin
Label protocol RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (Affymetrix, Santa Clara, CA) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
 
Hybridization protocol Standard hybridization to Affymetrix 430_2.0 arrays.
Scan protocol Standard scanning of Affymetrix 430_2.0 arrays.
Description Foxp3(gfp/gfp) homozygous female mice bear a GFP-Foxp3 fusion protein knockin allele, allowing for the visualization of regulatory T cells due to GFP-Foxp3 protein fluorescence.
Data processing GCMRA, Bioconductor
 
Submission date May 09, 2007
Last update date Aug 28, 2018
Contact name Alexander Rudensky
Organization name University of Washington
Department Immunology
Street address 1959 Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL1261
Series (2)
GSE7770 Gene expression in thymic CD4 T cells: effects of Foxp3
GSE7773 Foxp3-dependent programme of regulatory T-cell differentiation
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Thymic TN cells

Data table
ID_REF VALUE
1415670_at 7.899243702
1415671_at 6.557345386
1415672_at 9.322905689
1415673_at 5.480565635
1415674_a_at 5.01933626
1415675_at 5.172448496
1415676_a_at 6.858080103
1415677_at 3.910876926
1415678_at 6.433764571
1415679_at 9.459722547
1415680_at 6.94662549
1415681_at 5.416717493
1415682_at 3.982283122
1415683_at 9.462541117
1415684_at 3.81859453
1415685_at 7.130177367
1415686_at 5.352604188
1415687_a_at 8.521472675
1415688_at 7.319505779
1415689_s_at 6.091753507

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM188246.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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