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Sample GSM188247 Query DataSets for GSM188247
Status Public on May 18, 2007
Title GFP- CD25+ CD4+ CD8- thymocytes from Foxp3(gfp/gfp) mice
Sample type RNA
 
Source name thymocytes
Organism Mus musculus
Characteristics Strain: C57BL/6 backcross 4-6 from 129/Sv Gender: Female Age: 6-8 weeks
Treatment protocol Cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy
Label biotin
Label protocol RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (Affymetrix, Santa Clara, CA) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
 
Hybridization protocol Standard hybridization to Affymetrix 430_2.0 arrays.
Scan protocol Standard scanning of Affymetrix 430_2.0 arrays.
Description Foxp3(gfp/gfp) homozygous female mice bear a GFP-Foxp3 fusion protein knockin allele, allowing for the visualization of regulatory T cells due to GFP-Foxp3 protein fluorescence.
Data processing GCMRA, Bioconductor
 
Submission date May 09, 2007
Last update date Aug 28, 2018
Contact name Alexander Rudensky
Organization name University of Washington
Department Immunology
Street address 1959 Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL1261
Series (2)
GSE7770 Gene expression in thymic CD4 T cells: effects of Foxp3
GSE7773 Foxp3-dependent programme of regulatory T-cell differentiation
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Thymic T25 cells

Data table
ID_REF VALUE
1415670_at 7.400432805
1415671_at 4.745904181
1415672_at 8.881549368
1415673_at 5.428669499
1415674_a_at 5.531125311
1415675_at 5.18962728
1415676_a_at 6.579997456
1415677_at 3.915895219
1415678_at 4.97995435
1415679_at 8.346524111
1415680_at 6.559802629
1415681_at 5.418157663
1415682_at 3.993751546
1415683_at 9.421782644
1415684_at 3.733129107
1415685_at 7.871310973
1415686_at 4.966926457
1415687_a_at 7.038643375
1415688_at 7.562736387
1415689_s_at 3.083382637

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM188247.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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