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Sample GSM188248 Query DataSets for GSM188248
Status Public on May 18, 2007
Title GFP+ CD4+ CD8- thymocytes from Foxp3(gfpko/wt) mice
Sample type RNA
 
Source name thymocytes
Organism Mus musculus
Characteristics Strain: C57BL/6 backcross 2 from 129/Sv
Gender: Female Age: 6-8 weeks
Treatment protocol Cells were isolated by magnetic bead sorting followed by fluorescence activated cell sorting.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy
Label biotin
Label protocol RNA was converted to biotinylated cRNA (2 rounds of amplification) using commercially available reagent kits (Affymetrix, Santa Clara, CA) and were hybridized to Affymetrix mouse 430 2.0 microarrays.
 
Hybridization protocol Standard hybridization to Affymetrix 430_2.0 arrays.
Scan protocol Standard scanning of Affymetrix 430_2.0 arrays.
Description Foxp3(gfp/gfp) homozygous female mice bear a GFP-Foxp3 fusion protein knockin allele, allowing for the visualization of regulatory T cells due to GFP-Foxp3 protein fluorescence.
Data processing GCMRA, Bioconductor
 
Submission date May 09, 2007
Last update date Aug 28, 2018
Contact name Alexander Rudensky
Organization name University of Washington
Department Immunology
Street address 1959 Pacific St
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL1261
Series (2)
GSE7770 Gene expression in thymic CD4 T cells: effects of Foxp3
GSE7773 Foxp3-dependent programme of regulatory T-cell differentiation
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Thymic TFN cells

Data table
ID_REF VALUE
1415670_at 6.728574809
1415671_at 6.281852557
1415672_at 9.111179334
1415673_at 5.592460276
1415674_a_at 5.181400651
1415675_at 5.299269145
1415676_a_at 8.130211878
1415677_at 4.221855301
1415678_at 6.410590268
1415679_at 9.4461727
1415680_at 7.127064791
1415681_at 5.403366249
1415682_at 4.013041673
1415683_at 9.712685532
1415684_at 3.815179711
1415685_at 6.536102167
1415686_at 6.132080864
1415687_a_at 7.705794916
1415688_at 7.63432643
1415689_s_at 4.688588233

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM188248.CEL.gz 3.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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