|
| Status |
Public on Apr 01, 2009 |
| Title |
ES cells ctrl (GLUT4) RNAi rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse ES treated with lentiviral ctrl (GLUT4) RNAi
|
| Organism |
Mus musculus |
| Characteristics |
Mouse R1 cells
|
| Treatment protocol |
Cells were harvested 67h after lentiviral infection
|
| Growth protocol |
Cells were grown according to ATCC guidelines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen) according to manufacturers protocol
|
| Label |
Biotin
|
| Label protocol |
5 micrograms of RNA was RTed using Superscript from Invitrogen and labelled using the RNA transcript labelling kit from Enzo Life Science
|
| |
|
| Hybridization protocol |
according to standard Affymetrix protocol
|
| Scan protocol |
according to standard Affymetrix protocol
|
| Description |
shRNA targeting GLUT4 (as a control)
|
| Data processing |
GCOS using standard parameters
|
| |
|
| Submission date |
May 14, 2007 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Matthias D Kaeser |
| E-mail(s) |
kaeser@salk.edu
|
| Organization name |
The Salk Institute
|
| Department |
RBIO-E
|
| Street address |
10010 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE7791 |
Brd7, a novel PBAF-specific SWI/SNF subunit, is required for gene activation and repression in embryonic stem cells |
|
