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Sample GSM189606 Query DataSets for GSM189606
Status Public on Oct 01, 2007
Title B6 day 1, biological rep 2, tech rep 1
Sample type RNA
 
Source name Whole brain day 1
Organism Mus musculus
Characteristics C57/B6
Adult, male
Treatment protocol Cryopreserved P. berghei ANKA was thawed and passaged through naïve B6 donor mice until parasitemia in the passage animals reached approximately 10%. On day 0, mice were infected by intraperitoneal injection with 500,000 freshly isolated PbA parasitized erythrocytes. Parasitemia was monitored daily after Day 3 using thin blood smears
Growth protocol All adult male mice were housed in the same SPF facility.
Extracted molecule total RNA
Extraction protocol Brains were homogenized in Trizol Reagent and total RNA was isolated according to the manufacturers' instructions. RNA was further purified of gDNA contamination and concentrated using a RNeasyPlus Mini Kit.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
 
Hybridization protocol Following fragmentation, cRNA was hybridized to GeneChip MOE 430A 2.0 Array per Affymetrix protocols.
Scan protocol Microarrays were scanned using Affymetrix GeneChip Scanner and GeneChip Operating Software (GCOS) was used for image analysis.
Description Gene expression data from whole brain infected mouse, day 1
Data processing Background adjustment and quantile normalization across all microarrays was performed using the Robust Multichip Average algorithm (RMAExpress).
 
Submission date May 15, 2007
Last update date Oct 19, 2022
Contact name Sina Gharib
E-mail(s) sagharib@uw.edu
Organization name University of Washington
Department Medicine
Street address 850 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL8321
Series (1)
GSE7814 Transcriptional Profiling of Cerebral Malaria

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity.

Data table
ID_REF VALUE
1415670_at 355.1444181
1415671_at 1833.89713
1415672_at 1269.756373
1415673_at 136.373451
1415674_a_at 649.8315592
1415675_at 284.0868023
1415676_a_at 1294.954776
1415677_at 555.4918751
1415678_at 1010.832332
1415679_at 1338.237999
1415680_at 170.3549431
1415681_at 670.6401281
1415682_at 173.1696148
1415683_at 782.3790884
1415684_at 116.3171487
1415685_at 160.4592334
1415686_at 709.5649809
1415687_a_at 3743.217076
1415688_at 513.7280365
1415689_s_at 63.51000594

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM189606.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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