|
| Status |
Public on Oct 01, 2007 |
| Title |
B6 day 3, biological rep 3, tech rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Whole brain day 3
|
| Organism |
Mus musculus |
| Characteristics |
C57/B6
Adult, male
|
| Treatment protocol |
Cryopreserved P. berghei ANKA was thawed and passaged through naïve B6 donor mice until parasitemia in the passage animals reached approximately 10%. On day 0, mice were infected by intraperitoneal injection with 500,000 freshly isolated PbA parasitized erythrocytes. Parasitemia was monitored daily after Day 3 using thin blood smears
|
| Growth protocol |
All adult male mice were housed in the same SPF facility.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Brains were homogenized in Trizol Reagent and total RNA was isolated according to the manufacturers' instructions. RNA was further purified of gDNA contamination and concentrated using a RNeasyPlus Mini Kit.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized to GeneChip MOE 430A 2.0 Array per Affymetrix protocols.
|
| Scan protocol |
Microarrays were scanned using Affymetrix GeneChip Scanner and GeneChip Operating Software (GCOS) was used for image analysis.
|
| Description |
Gene expression data from whole brain infected mouse, day 3
|
| Data processing |
Background adjustment and quantile normalization across all microarrays was performed using the Robust Multichip Average algorithm (RMAExpress).
|
| |
|
| Submission date |
May 15, 2007 |
| Last update date |
Oct 19, 2022 |
| Contact name |
Sina Gharib |
| E-mail(s) |
sagharib@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Medicine
|
| Street address |
850 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE7814 |
Transcriptional Profiling of Cerebral Malaria |
|
