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Sample GSM189637 Query DataSets for GSM189637
Status Public on Oct 01, 2007
Title Balb/c day 1, biological rep 1, tech rep 2
Sample type RNA
 
Source name Whole brain day 1
Organism Mus musculus
Characteristics Balb/C
Adult, male
Treatment protocol Cryopreserved P. berghei ANKA was thawed and passaged through naïve B6 donor mice until parasitemia in the passage animals reached approximately 10%. On day 0, mice were infected by intraperitoneal injection with 500,000 freshly isolated PbA parasitized erythrocytes. Parasitemia was monitored daily after Day 3 using thin blood smears
Growth protocol All adult male mice were housed in the same SPF facility.
Extracted molecule total RNA
Extraction protocol Brains were homogenized in Trizol Reagent and total RNA was isolated according to the manufacturers' instructions. RNA was further purified of gDNA contamination and concentrated using a RNeasyPlus Mini Kit.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
 
Hybridization protocol Following fragmentation, cRNA was hybridized to GeneChip MOE 430A 2.0 Array per Affymetrix protocols.
Scan protocol Microarrays were scanned using Affymetrix GeneChip Scanner and GeneChip Operating Software (GCOS) was used for image analysis.
Description Gene expression data from whole brain infected mouse, day 1
Data processing Background adjustment and quantile normalization across all microarrays was performed using the Robust Multichip Average algorithm (RMAExpress).
 
Submission date May 15, 2007
Last update date Oct 19, 2022
Contact name Sina Gharib
E-mail(s) sagharib@uw.edu
Organization name University of Washington
Department Medicine
Street address 850 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL8321
Series (1)
GSE7814 Transcriptional Profiling of Cerebral Malaria

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity.

Data table
ID_REF VALUE
1415670_at 385.1690578
1415671_at 1741.303544
1415672_at 1149.174365
1415673_at 86.71904717
1415674_a_at 520.1002784
1415675_at 262.5180843
1415676_a_at 1224.8828
1415677_at 538.7626394
1415678_at 1095.911817
1415679_at 1156.462684
1415680_at 167.7576794
1415681_at 641.4668941
1415682_at 169.0331033
1415683_at 879.1332252
1415684_at 132.6882674
1415685_at 127.5034081
1415686_at 639.5520644
1415687_a_at 3652.435296
1415688_at 537.3450553
1415689_s_at 69.80831579

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM189637.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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