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Sample GSM189643 Query DataSets for GSM189643
Status Public on Oct 01, 2007
Title Balb/c day 1, biological rep 4, tech rep 2
Sample type RNA
 
Source name Whole brain day 1
Organism Mus musculus
Characteristics Balb/C
Adult, male
Treatment protocol Cryopreserved P. berghei ANKA was thawed and passaged through naïve B6 donor mice until parasitemia in the passage animals reached approximately 10%. On day 0, mice were infected by intraperitoneal injection with 500,000 freshly isolated PbA parasitized erythrocytes. Parasitemia was monitored daily after Day 3 using thin blood smears
Growth protocol All adult male mice were housed in the same SPF facility.
Extracted molecule total RNA
Extraction protocol Brains were homogenized in Trizol Reagent and total RNA was isolated according to the manufacturers' instructions. RNA was further purified of gDNA contamination and concentrated using a RNeasyPlus Mini Kit.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
 
Hybridization protocol Following fragmentation, cRNA was hybridized to GeneChip MOE 430A 2.0 Array per Affymetrix protocols.
Scan protocol Microarrays were scanned using Affymetrix GeneChip Scanner and GeneChip Operating Software (GCOS) was used for image analysis.
Description Gene expression data from whole brain infected mouse, day 1
Data processing Background adjustment and quantile normalization across all microarrays was performed using the Robust Multichip Average algorithm (RMAExpress).
 
Submission date May 15, 2007
Last update date Oct 19, 2022
Contact name Sina Gharib
E-mail(s) sagharib@uw.edu
Organization name University of Washington
Department Medicine
Street address 850 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL8321
Series (1)
GSE7814 Transcriptional Profiling of Cerebral Malaria

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity.

Data table
ID_REF VALUE
1415670_at 327.7818134
1415671_at 1626.02982
1415672_at 962.1159757
1415673_at 99.44548731
1415674_a_at 516.946439
1415675_at 280.3746298
1415676_a_at 1248.862561
1415677_at 499.1006595
1415678_at 1011.568989
1415679_at 1207.321341
1415680_at 146.482753
1415681_at 676.5899794
1415682_at 172.4598835
1415683_at 705.4754164
1415684_at 112.8788981
1415685_at 132.2853969
1415686_at 686.1865321
1415687_a_at 3246.618156
1415688_at 374.6573645
1415689_s_at 62.82265456

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM189643.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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