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Status |
Public on Jun 07, 2007 |
Title |
Vehicle control (5.25% glucose) for 24 h_6 |
Sample type |
RNA |
|
|
Source name |
CL from female bonnet monkey 24 h post VEH (5.25% glucose)
|
Organism |
Macaca radiata |
Characteristics |
CL collected from female bonnet monkey 24 h post VEH (5.25% glucose)
|
Biomaterial provider |
R. Medhamurthy
|
Treatment protocol |
A single s.c. injection of VEH (5.25% glucose) was administered to female monkeys on day 7 of luteal phase and CL collected at 24 h post VEH injection was cut into small quarters and snap frozen in liquid nitrogen.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Trizol reagent (Sigma Aldrich Co., St.Louis, MO) followed by clean up step using RNeasy mini purification kit (# 74104, Qiagen).
|
Label |
Biotin
|
Label protocol |
Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev.3, 2001)
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|
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Hybridization protocol |
Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev.3, 2001)
|
Scan protocol |
Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev.3, 2001) using scanner 3000 series
|
Description |
Probe level measurements produced by Affymetrix microarray suite 5.0. Analysis performed at level of raw signals (See Affymetrix Microarray suite 5.0 reference on web site for description).
|
Data processing |
MAS5.0, data was normalized with standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev.3, 2001)
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|
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Submission date |
May 17, 2007 |
Last update date |
Jun 06, 2007 |
Contact name |
priyanka samji |
E-mail(s) |
priyanka@mrdg.iisc.ernet.in
|
Phone |
91-80-22932490
|
Fax |
91-80-23600999
|
Organization name |
Indian Institute of Science
|
Department |
Molecular Reproduction Development and Genetics
|
Lab |
R. Medhamurthy's lab
|
Street address |
Malleswaram
|
City |
Bangalore |
State/province |
Karnataka |
ZIP/Postal code |
560012 |
Country |
India |
|
|
Platform ID |
GPL3535 |
Series (1) |
GSE7827 |
Identification of luteinizing hormone regulated genes in the primate corpus luteum |
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