|
| Status |
Public on Oct 31, 2007 |
| Title |
eutopic endometrium from patients with endometriosis, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
endometrial endothelial cell from eutopic endometrium
|
| Organism |
Homo sapiens |
| Characteristics |
endothelium from a patient with endometriosis
|
| Biomaterial provider |
Guihua Sha
|
| Treatment protocol |
primary in vitro culture followed by two passages.
|
| Growth protocol |
DMEM with 20% FBS, 100 ug/ml ECGS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to instruction manual of Single Round aRNA Amplification Kit from 2 microg total RNA (MessageAmp? II-Bintin Enhaned KIT, Ambion).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the genechip Scanner 3000.
|
| Description |
Gene expression data from ectopic endometrium
|
| Data processing |
A global scaling procedure was performed to normalize the different arrays using dChip software. In a comparison analysis, we applied two class unpaired method in the SAM (Significant Analysis of Microarray ) software to identify significantly differentially expressed genes between the EM group and non-EM group.
|
| |
|
| Submission date |
May 18, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Guihua Sha |
| E-mail(s) |
shaguihua1970@yahoo.com.cn
|
| Organization name |
Peking Union Medical College Hospital
|
| Street address |
1 Wangfujing, Shuaifuyuan
|
| City |
BeiJing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7846 |
Differentially expressed genes in HEECs of eutopic endometrium of patients with endometriosis compared with control |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
