|
| Status |
Public on Jun 11, 2008 |
| Title |
Distinctive molecular signature of membrane type-1 matrix metalloproteinase (MT1-MMP) in cancer: HT_siRNA rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ATCC; in house cell culture
|
| Organism |
Homo sapiens |
| Characteristics |
The parental human fibrosarcoma HT1080 (HT cells) were obtained from ATCC
|
| Biomaterial provider |
ATCC; in house cell culture
|
| Treatment protocol |
HT cells were stably transfected with the wild type MT1-MMP, MT1-MMP siRNA and scrambled siRNA using LipofectAMINE (Invitrogen) to generate HT-MT, HT-siRNA and HTsiRNAscr cells, respectively.
|
| Growth protocol |
HT and U cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (DMEM/FCS) and 10 μg/ml gentamicin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Illumina total prep RNA amplification kit (Ambion catalog#IL1791)
|
| Label |
Streptavidin Cy3
|
| Label protocol |
As described in the illumina human Ref8 chip processing protocol. Briefly generation of biotin labelled cRNA from the product of RT cDNA synthesis and 2nd strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
As described in the illumina human ref8 hybridisation protocol. Doc#11176837 Rev E.
|
| Scan protocol |
As suggested by Illumina using the illumina scanner
|
| Description |
HT_siRNA rep1: 1st biological replicate of 2.
|
| Data processing |
none: raw data from Beadstudio
|
| |
|
| Submission date |
May 18, 2007 |
| Last update date |
Jun 10, 2008 |
| Contact name |
Roy M Williams |
| E-mail(s) |
rwillia@scripps.edu
|
| Organization name |
The Scripps Research Institute
|
| Department |
Regenerative Medicine
|
| Lab |
Loring
|
| Street address |
3030 Science Park Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL2700 |
| Series (1) |
| GSE11668 |
Molecular Signature of MT1-MMP: Transactivation of the Downstream Universal Gene in Cancer |
|
