|
Status |
Public on Jun 11, 2008 |
Title |
Distinctive molecular signature of membrane type-1 matrix metalloproteinase (MT1-MMP) in cancer: HT-siRNA rep2 |
Sample type |
RNA |
|
|
Source name |
ATCC; in house cell culture
|
Organism |
Homo sapiens |
Characteristics |
Transfected HT0810 cell line
|
Biomaterial provider |
ATCC; in house cell culture
|
Treatment protocol |
HT cells were stably transfected with the wild type MT1-MMP, MT1-MMP siRNA and scrambled siRNA using LipofectAMINE (Invitrogen) to generate HT-MT, HT-siRNA and HTsiRNAscr cells, respectively.
|
Growth protocol |
HT and U cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (DMEM/FCS) and 10 μg/ml gentamicin.
|
Extracted molecule |
total RNA |
Extraction protocol |
Illumina total prep RNA amplification kit (Ambion catalog#IL1791)
|
Label |
Streptavidin Cy3
|
Label protocol |
As described in the illumina human Ref8 chip processing protocol. Briefly generation of biotin labelled cRNA from the product of RT cDNA synthesis and 2nd strand cDNA synthesis.
|
|
|
Hybridization protocol |
As described in the illumina human ref8 hybridisation protocol. Doc#11176837 Rev E.
|
Scan protocol |
As suggested by Illumina using the illumina scanner
|
Description |
HT-siRNA rep 2: 2nd biological replicate of 2.
|
Data processing |
none: raw data from Beadstudio
|
|
|
Submission date |
May 18, 2007 |
Last update date |
Jun 10, 2008 |
Contact name |
Roy M Williams |
E-mail(s) |
rwillia@scripps.edu
|
Organization name |
The Scripps Research Institute
|
Department |
Regenerative Medicine
|
Lab |
Loring
|
Street address |
3030 Science Park Road
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL2700 |
Series (1) |
GSE11668 |
Molecular Signature of MT1-MMP: Transactivation of the Downstream Universal Gene in Cancer |
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