|
Status |
Public on Mar 01, 2009 |
Title |
Gastrocnemius Muscle Galgt2 Transgenic rep2 |
Sample type |
RNA |
|
|
Source name |
Gastrocnemius, 2 months old
|
Organism |
Mus musculus |
Characteristics |
Strain: C57BL/6J, Gender: Male, Age: 2 months, Tissue: Gastrocnemius Muscle. Galgt2 Transgenic
|
Treatment protocol |
1AAV-Galgt2 and 2AAV-Galgt2 were mdx mice whose gastrocnemius muscle was treated with AAV carrying the Galgt2 gene. 3mdxPBS and 4mdxPBS were contralateral muscles mock-treated with phosphate-buffered saline.
|
Growth protocol |
All mice were maintained in the institute animal facility following the IACUC guidelines
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by further purification on a silica-gel-based membrane (RNeasy-Mini; Qiagen, Valencia, CA).
|
Label |
Biotin
|
Label protocol |
The Ovation™ Biotin RNA Amplification and Labeling System (NuGen Technologies, Inc., San Carlos, CA) was used to prepare amplified, biotin-labeled cDNA from 25 ng of total RNA from gastrocnemius muscle following manufacturer’s instructions.
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|
|
Hybridization protocol |
For each array, 2.2 µg of fragmented and labeled cDNA was hybridized onto 430 2.0 GeneChips® (Affymetrix Inc., USA), for 16 hours at 45ºC followed by washing and staining of microarrays in a Fluidics Station 450 (Affymetrix Inc., USA).
|
Scan protocol |
GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix Inc., USA).
|
Description |
Gene expression data from skeletal muscle of two months old mouse
|
Data processing |
CEL files were generated from DAT files using GeneChip® Operating Software (GCOS) software (Affymetrix Inc., USA) and probe set signals were generated using the RMA algorithm in ArrayAssist 3.4 (Stratagene).
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|
|
Submission date |
May 21, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Paul T Martin |
E-mail(s) |
MartinPT@pediatrics.ohio-state.edu
|
Organization name |
Columbus Children's Research Institute
|
Street address |
700 Children's Drive
|
City |
Columbus |
State/province |
OH |
ZIP/Postal code |
43205 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7863 |
Gene expression profiling of Galgt2 overexpression in mdx skeletal muscle |
|
Relations |
Reanalyzed by |
GSE119085 |