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Sample GSM190640 Query DataSets for GSM190640
Status Public on Mar 01, 2009
Title Gastrocnemius Muscle Galgt2 Transgenic rep2
Sample type RNA
 
Source name Gastrocnemius, 2 months old
Organism Mus musculus
Characteristics Strain: C57BL/6J, Gender: Male, Age: 2 months, Tissue: Gastrocnemius Muscle. Galgt2 Transgenic
Treatment protocol 1AAV-Galgt2 and 2AAV-Galgt2 were mdx mice whose gastrocnemius muscle was treated with AAV carrying the Galgt2 gene. 3mdxPBS and 4mdxPBS were contralateral muscles mock-treated with phosphate-buffered saline.
Growth protocol All mice were maintained in the institute animal facility following the IACUC guidelines
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by further purification on a silica-gel-based membrane (RNeasy-Mini; Qiagen, Valencia, CA).
Label Biotin
Label protocol The Ovation™ Biotin RNA Amplification and Labeling System (NuGen Technologies, Inc., San Carlos, CA) was used to prepare amplified, biotin-labeled cDNA from 25 ng of total RNA from gastrocnemius muscle following manufacturer’s instructions.
 
Hybridization protocol For each array, 2.2 µg of fragmented and labeled cDNA was hybridized onto 430 2.0 GeneChips® (Affymetrix Inc., USA), for 16 hours at 45ºC followed by washing and staining of microarrays in a Fluidics Station 450 (Affymetrix Inc., USA).
Scan protocol GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix Inc., USA).
Description Gene expression data from skeletal muscle of two months old mouse
Data processing CEL files were generated from DAT files using GeneChip® Operating Software (GCOS) software (Affymetrix Inc., USA) and probe set signals were generated using the RMA algorithm in ArrayAssist 3.4 (Stratagene).
 
Submission date May 21, 2007
Last update date Aug 28, 2018
Contact name Paul T Martin
E-mail(s) MartinPT@pediatrics.ohio-state.edu
Organization name Columbus Children's Research Institute
Street address 700 Children's Drive
City Columbus
State/province OH
ZIP/Postal code 43205
Country USA
 
Platform ID GPL1261
Series (1)
GSE7863 Gene expression profiling of Galgt2 overexpression in mdx skeletal muscle
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Probe set signals were generated using the RMA algorithm in ArrayAssist 3.4 (Stratagene) and differential gene expression was determined by pair-wise comparisons.

Data table
ID_REF VALUE
1415670_at 501.761397
1415671_at 823.013567
1415672_at 1256.991628
1415673_at 305.011387
1415674_a_at 446.54556
1415675_at 518.969825
1415676_a_at 6736.653666
1415677_at 558.310772
1415678_at 7255.707702
1415679_at 2323.219028
1415680_at 387.116434
1415681_at 981.914927
1415682_at 536.245019
1415683_at 1378.876984
1415684_at 261.746426
1415685_at 672.361788
1415686_at 4056.000513
1415687_a_at 4758.357236
1415688_at 3877.263693
1415689_s_at 155.986973

Total number of rows: 45101

Table truncated, full table size 957 Kbytes.




Supplementary file Size Download File type/resource
GSM190640.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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