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Sample GSM190790 Query DataSets for GSM190790
Status Public on Jul 01, 2007
Title PP M CELL2
Sample type RNA
 
Source name PP M CELL
Organism Mus musculus
Characteristics Mouse Peyer's patch M cell
Extracted molecule total RNA
Extraction protocol RNA was isolated with a High Pure RNA Tissue Kit (Roche, Penzberg, Germany) according to the manufacturer’s instructions.
Label Biotin
Label protocol Biotinylated cRNA was prepared using a two-cycle target labeling assay in accordance with the protocol of the manufacture (Affymetrix, Santa Clara, CA, USA).
 
Hybridization protocol The cRNA was hybridized with DNA probes on a GeneChip Mouse Genome 430 2.0 Array (Affymetrix), washed, and fluorescence-labeled in accordance with the standard amplification protocol for eukaryotic targets developed by Affymetrix.
Scan protocol The arrays were scanned with a GeneChip Scanner 3000 7G (Affymetrix). The fluorescence intensity of each probe was taken to represent the raw expression level and was quantified using GeneChip Operating software (Affymetrix).
Description Epithelial cells in duodenal Peyer's patches (PP) of naive BALB/c mice were stained with NKM16-2-4 monoclonal antibody (mAb)-FITC, lectin UEA-1-PE, and anti-CD45 mAb-APC-Cy7. PP M cells (NKM16-2-4+, UEA-1+, and CD45- cells) were isolated by FACS.
Data processing The fluorescence intensity of each probe was taken to represent the raw expression level and was quantified using GeneChip Operating Software (Affymetrix).
 
Submission date May 21, 2007
Last update date Aug 28, 2018
Contact name Kazutaka Terahara
E-mail(s) tera@nih.go.jp
Organization name The University of Tokyo
Department Institute of Medical Science
Street address 4-6-1 Shirokanedai
City Minato-ku
ZIP/Postal code 108-8639
Country Japan
 
Platform ID GPL1261
Series (1)
GSE7838 Comprehensive Gene ExpressionProfiling of Peyer’s Patch M Cells, Villous M-like Cells, and Intestinal Epithelial Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RAW VALUE
ABS_CALL ABSENT PRESENT MARGINAL
DETECTION P-VALUE P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 209.8 P 0.000095
AFFX-BioB-M_at 345.3 P 0.000081
AFFX-BioB-3_at 197.2 P 0.000044
AFFX-BioC-5_at 551.3 P 0.000052
AFFX-BioC-3_at 553.5 P 0.000044
AFFX-BioDn-5_at 1113.4 P 0.000044
AFFX-BioDn-3_at 1829.6 P 0.000044
AFFX-CreX-5_at 6496.2 P 0.000052
AFFX-CreX-3_at 6584.1 P 0.000044
AFFX-DapX-5_at 105.8 P 0.000127
AFFX-DapX-M_at 1063.9 P 0.000081
AFFX-DapX-3_at 2065.3 P 0.000044
AFFX-LysX-5_at 26.2 P 0.000662
AFFX-LysX-M_at 138.7 P 0.000044
AFFX-LysX-3_at 287.1 P 0.000044
AFFX-PheX-5_at 54.3 P 0.000225
AFFX-PheX-M_at 204.6 P 0.000509
AFFX-PheX-3_at 324.7 P 0.000195
AFFX-ThrX-5_at 25 P 0.033677
AFFX-ThrX-M_at 162 P 0.000044

Total number of rows: 45101

Table truncated, full table size 1188 Kbytes.




Supplementary file Size Download File type/resource
GSM190790.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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