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Sample GSM190794 Query DataSets for GSM190794
Status Public on Jul 01, 2007
Title CT-v-M cell3
Sample type RNA
 
Source name CT-v-M cell
Organism Mus musculus
Characteristics Cholera toxin-induced villous M cell
Extracted molecule total RNA
Extraction protocol RNA was isolated with a High Pure RNA Tissue Kit (Roche, Penzberg, Germany) according to the manufacturer’s instructions.
Label Biotin
Label protocol Biotinylated cRNA was prepared using a two-cycle target labeling assay in accordance with the protocol of the manufacture (Affymetrix, Santa Clara, CA, USA).
 
Hybridization protocol The cRNA was hybridized with DNA probes on a GeneChip Mouse Genome 430 2.0 Array (Affymetrix), washed, and fluorescence-labeled in accordance with the standard amplification protocol for eukaryotic targets developed by Affymetrix.
Scan protocol The arrays were scanned with a GeneChip Scanner 3000 7G (Affymetrix). The fluorescence intensity of each probe was taken to represent the raw expression level and was quantified using GeneChip Operating software (Affymetrix).
Description Epithelial cells in duodenal villi of CT-administered BALB/c mice were stained with NKM16-2-4 monoclonal antibody (mAb)-FITC, lectin UEA-1-PE, and anti-CD45 mAb-APC-Cy7. Induced villous M cells (NKM16-2-4+, UEA-1+, and CD45- cells) were isolated by FACS.
Data processing The fluorescence intensity of each probe was taken to represent the raw expression level and was quantified using GeneChip Operating Software (Affymetrix).
 
Submission date May 21, 2007
Last update date Aug 28, 2018
Contact name Kazutaka Terahara
E-mail(s) tera@nih.go.jp
Organization name The University of Tokyo
Department Institute of Medical Science
Street address 4-6-1 Shirokanedai
City Minato-ku
ZIP/Postal code 108-8639
Country Japan
 
Platform ID GPL1261
Series (1)
GSE7838 Comprehensive Gene ExpressionProfiling of Peyer’s Patch M Cells, Villous M-like Cells, and Intestinal Epithelial Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RAW VALUE
ABS_CALL ABSENT PRESENT MARGINAL
DETECTION P-VALUE P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 191.9 P 0.00034
AFFX-BioB-M_at 233.4 P 0.000044
AFFX-BioB-3_at 202 P 0.000044
AFFX-BioC-5_at 560.6 P 0.000052
AFFX-BioC-3_at 456.4 P 0.000052
AFFX-BioDn-5_at 994.9 P 0.000044
AFFX-BioDn-3_at 2004.1 P 0.000044
AFFX-CreX-5_at 6186.2 P 0.000044
AFFX-CreX-3_at 5837.2 P 0.000044
AFFX-DapX-5_at 98.7 P 0.00007
AFFX-DapX-M_at 846 P 0.00006
AFFX-DapX-3_at 1490.3 P 0.000044
AFFX-LysX-5_at 22 P 0.00034
AFFX-LysX-M_at 142.8 P 0.00007
AFFX-LysX-3_at 285.7 P 0.000052
AFFX-PheX-5_at 72.5 P 0.000297
AFFX-PheX-M_at 155.7 P 0.00011
AFFX-PheX-3_at 269 P 0.000297
AFFX-ThrX-5_at 36.7 P 0.000857
AFFX-ThrX-M_at 149.6 P 0.000044

Total number of rows: 45101

Table truncated, full table size 1178 Kbytes.




Supplementary file Size Download File type/resource
GSM190794.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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