NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM190797 Query DataSets for GSM190797
Status Public on Jul 01, 2007
Title IEC3
Sample type RNA
 
Source name IEC
Organism Mus musculus
Characteristics Intestinal epithelial cell (IEC)
Extracted molecule total RNA
Extraction protocol RNA was isolated with a High Pure RNA Tissue Kit (Roche, Penzberg, Germany) according to the manufacturer’s instructions.
Label Biotin
Label protocol Biotinylated cRNA was prepared using a two-cycle target labeling assay in accordance with the protocol of the manufacture (Affymetrix, Santa Clara, CA, USA).
 
Hybridization protocol The cRNA was hybridized with DNA probes on a GeneChip Mouse Genome 430 2.0 Array (Affymetrix), washed, and fluorescence-labeled in accordance with the standard amplification protocol for eukaryotic targets developed by Affymetrix.
Scan protocol The arrays were scanned with a GeneChip Scanner 3000 7G (Affymetrix). The fluorescence intensity of each probe was taken to represent the raw expression level and was quantified using GeneChip Operating software (Affymetrix).
Description Epithelial cells in duodenal villi of naive BALB/c mice were stained with NKM16-2-4 monoclonal antibody (mAb)-FITC, lectin UEA-1-PE, and anti-CD45 mAb-APC-Cy7. IECs (NKM16-2-4-, UEA-1-, and CD45- cells) were isolated by FACS.
Data processing The fluorescence intensity of each probe was taken to represent the raw expression level and was quantified using GeneChip Operating Software (Affymetrix).
 
Submission date May 21, 2007
Last update date Aug 28, 2018
Contact name Kazutaka Terahara
E-mail(s) tera@nih.go.jp
Organization name The University of Tokyo
Department Institute of Medical Science
Street address 4-6-1 Shirokanedai
City Minato-ku
ZIP/Postal code 108-8639
Country Japan
 
Platform ID GPL1261
Series (1)
GSE7838 Comprehensive Gene ExpressionProfiling of Peyer’s Patch M Cells, Villous M-like Cells, and Intestinal Epithelial Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RAW VALUE
ABS_CALL ABSENT PRESENT MARGINAL
DETECTION P-VALUE P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 188.8 P 0.000581
AFFX-BioB-M_at 260.2 P 0.00006
AFFX-BioB-3_at 179.4 P 0.000052
AFFX-BioC-5_at 550.4 P 0.000052
AFFX-BioC-3_at 555 P 0.000044
AFFX-BioDn-5_at 1085.6 P 0.000044
AFFX-BioDn-3_at 1745.9 P 0.000044
AFFX-CreX-5_at 6188.7 P 0.000052
AFFX-CreX-3_at 6326.7 P 0.000044
AFFX-DapX-5_at 98.4 P 0.00006
AFFX-DapX-M_at 847.7 P 0.000081
AFFX-DapX-3_at 1638.7 P 0.000044
AFFX-LysX-5_at 22.9 P 0.001102
AFFX-LysX-M_at 175.3 P 0.000195
AFFX-LysX-3_at 363.7 P 0.000052
AFFX-PheX-5_at 66.9 P 0.00007
AFFX-PheX-M_at 176.5 P 0.000081
AFFX-PheX-3_at 254 P 0.00007
AFFX-ThrX-5_at 42.8 P 0.002554
AFFX-ThrX-M_at 146.9 P 0.000044

Total number of rows: 45101

Table truncated, full table size 1184 Kbytes.




Supplementary file Size Download File type/resource
GSM190797.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap