|
| Status |
Public on Jul 01, 2007 |
| Title |
IEC3 |
| Sample type |
RNA |
| |
|
| Source name |
IEC
|
| Organism |
Mus musculus |
| Characteristics |
Intestinal epithelial cell (IEC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with a High Pure RNA Tissue Kit (Roche, Penzberg, Germany) according to the manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared using a two-cycle target labeling assay in accordance with the protocol of the manufacture (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
The cRNA was hybridized with DNA probes on a GeneChip Mouse Genome 430 2.0 Array (Affymetrix), washed, and fluorescence-labeled in accordance with the standard amplification protocol for eukaryotic targets developed by Affymetrix.
|
| Scan protocol |
The arrays were scanned with a GeneChip Scanner 3000 7G (Affymetrix). The fluorescence intensity of each probe was taken to represent the raw expression level and was quantified using GeneChip Operating software (Affymetrix).
|
| Description |
Epithelial cells in duodenal villi of naive BALB/c mice were stained with NKM16-2-4 monoclonal antibody (mAb)-FITC, lectin UEA-1-PE, and anti-CD45 mAb-APC-Cy7. IECs (NKM16-2-4-, UEA-1-, and CD45- cells) were isolated by FACS.
|
| Data processing |
The fluorescence intensity of each probe was taken to represent the raw expression level and was quantified using GeneChip Operating Software (Affymetrix).
|
| |
|
| Submission date |
May 21, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Kazutaka Terahara |
| E-mail(s) |
tera@nih.go.jp
|
| Organization name |
The University of Tokyo
|
| Department |
Institute of Medical Science
|
| Street address |
4-6-1 Shirokanedai
|
| City |
Minato-ku |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7838 |
Comprehensive Gene ExpressionProfiling of Peyer’s Patch M Cells, Villous M-like Cells, and Intestinal Epithelial Cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
