|
Status |
Public on Feb 14, 2017 |
Title |
pDC.CLN_3 |
Sample type |
RNA |
|
|
Source name |
Plasmacytoid dendritic cells_cutaneous lymph node
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: cutaneous lymph nodes cell markers: CD45(+)Lin(-)CD117(-)CD11b(-)CD11c(low)MHC-II(- to low)PDCA-1(+)B220(+)Ly6C(+) cell type: plasmacytoid dendritic cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Plasmacytoid dendritic cells were purified by flow cytometry and total RNA was extracted with the Qiagen micro RNAeasy kit, with on column DNase digestion, according to the manufacturer protocol.
|
Label |
biotin
|
Label protocol |
1 to 20 ng of RNA from each sample was used to synthesize biotinylated probes, using 2 successive rounds of cRNA amplification (using the NuGEN Ovation Pico WTA System V2 Kit and the NuGEN Encore Biotin Module Kit according to NuGEN recommendations) with appropriate quality control to ensure full length synthesis.
|
|
|
Hybridization protocol |
Following fragmentation and end-labeling, 2.07 μg of cDNAs were hybridized for 16 hours at 45°C on Affymetrix GeneChip Mouse Gene 1.0 ST arrays. The chips were washed and stained in the GeneChip Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm.
|
Description |
Untreated
|
Data processing |
The data were RMA normalized in R and Bioconductor, using the oligo package.
|
|
|
Submission date |
Oct 22, 2015 |
Last update date |
Feb 14, 2017 |
Contact name |
Marc DALOD |
E-mail(s) |
dalod@ciml.univ-mrs.fr
|
Organization name |
CIML
|
Street address |
Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, Case 906, 13288 Marseille cedex 9
|
City |
MARSEILLE |
ZIP/Postal code |
13009 |
Country |
France |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE74276 |
Gene expression profiling of plasmacytoid dendritic cells from cutaneous lymph nodes |
|
Relations |
Reanalyzed by |
GSE74316 |