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Sample GSM194074 Query DataSets for GSM194074
Status Public on May 25, 2007
Title 271_ureter
Sample type RNA
 
Source name ureter
Organism Mus musculus
Characteristics gene reported: SMGA/EGFP transgenic / PMID: 14713859
strain: FVB/N
sex: male
developmental stage: Adult
theiler stage: 28
somite count: N/A
developmental landmark: N/A-Adult
Treatment protocol pool size: N/A
Pooled sample: no
Dissection Method: Ureter was micro-dissected between the kidney and bladder based on the presence of SMGA/EGFP.
Female FVB/N mice were mated to SMGA/EGFP transgenic males and allowed to deliver. The SMGA/EGFP mice were euthanized by standard carbon dioxide asphyxiation at adulthood. Tissue samples were dissected, rapidly frozen and stored at -80 °C until RNA was extracted for gene expression analysis.
Extracted molecule total RNA
Extraction protocol Qiagen: RNeasy
Label Biotin
Label protocol Biotin-X-X-NHS (EPICENTRE Biotechnologies)
 
Hybridization protocol Affymetrix standard protocol
Amount labeled target hybridization to array: 10 ug
Scan protocol Affymetrix standard protocol
Description >> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter protocols
Rounds of amplification: 2
URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:7980
Data processing Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
 
Submission date May 24, 2007
Last update date Aug 28, 2018
Contact name GUDMAP Developers
E-mail(s) gudmap-db@gudmap.org
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE7887 Gene expression profiles of adult ureter isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 15)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 793.41 P 0.000225
AFFX-BioB-M_at 1499.74 P 0.000052
AFFX-BioB-3_at 796.23 P 0.000044
AFFX-BioC-5_at 1009.79 P 0.000060
AFFX-BioC-3_at 1474.33 P 0.000044
AFFX-BioDn-5_at 6617.36 P 0.000044
AFFX-BioDn-3_at 14175.68 P 0.000044
AFFX-CreX-5_at 36175.21 P 0.000052
AFFX-CreX-3_at 44132.45 P 0.000044
AFFX-DapX-5_at 57.12 P 0.002556
AFFX-DapX-M_at 4.89 A 0.760937
AFFX-DapX-3_at 5.63 A 0.843268
AFFX-LysX-5_at 1.55 A 0.876428
AFFX-LysX-M_at 7.04 A 0.574038
AFFX-LysX-3_at 40.57 M 0.050229
AFFX-PheX-5_at 2.21 A 0.995002
AFFX-PheX-M_at 3.53 A 0.927300
AFFX-PheX-3_at 117.33 A 0.078002
AFFX-ThrX-5_at 52.55 A 0.139482
AFFX-ThrX-M_at 13.21 A 0.470241

Total number of rows: 45101

Table truncated, full table size 1287 Kbytes.




Supplementary file Size Download File type/resource
GSM194074.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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