individual: Sarcoidosis Patient cell type: BAL cells
Treatment protocol
BAL cells were isolated from BAL fluid by centrifugation at 1,000 g for 10 minutes at 4°C. BAL cells were washed with hanks balanced salt solution, resuspended and counted with a hemocytometer. Mean viability of lavage cells was > 95% as determined by trypan blue dye exclusion.
Growth protocol
None
Extracted molecule
total RNA
Extraction protocol
RNeasy Mini Kit (Qiagen, Valencia, CA)
Label
Biotin
Label protocol
Affymetrix standard protocol
Hybridization protocol
Affymetrix standard protocol
Scan protocol
GeneChip Operating System (GCOS)
Data processing
RMA algorithm with quantile normalization and background correction
