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Sample GSM194324 Query DataSets for GSM194324
Status Public on Aug 09, 2008
Title Mouse Myc-induced lymphoma 11
Sample type RNA
 
Source name Lymphoma from Em-myc transgenic mouse
Organism Mus musculus
Characteristics Genotype: Em-myc transgenic
Onset of age: 61
Extracted molecule total RNA
Extraction protocol Snap-frozen samples in liquid nitrogen were used for extraction. Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 24, 2007
Last update date Aug 28, 2018
Contact name Seiichi Mori
E-mail(s) seiichi.mori@nus.edu.sg
Phone (65) 6516 1148
Fax (65) 6873 9664
URL http://www.csi.nus.edu.sg
Organization name National University of Singapore
Department Cancer Science Institute Singapore
Lab Genomic Oncology Programme
Street address Centre for Life Sciences #02-07, 28 Medical Drive
City Singapore
State/province Singapore
ZIP/Postal code 117456
Country Singapore
 
Platform ID GPL1261
Series (1)
GSE7897 Expression data from Mouse Lymphoma
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1308.701538
1415671_at 1589.540293
1415672_at 5660.077182
1415673_at 606.1568755
1415674_a_at 1193.617368
1415675_at 973.2564759
1415676_a_at 5071.200331
1415677_at 653.7197088
1415678_at 1916.541313
1415679_at 4349.044915
1415680_at 2790.829834
1415681_at 2548.385797
1415682_at 1563.472025
1415683_at 3929.105393
1415684_at 890.2039409
1415685_at 1187.82354
1415686_at 1703.008539
1415687_a_at 12573.14498
1415688_at 3120.221789
1415689_s_at 974.8202668

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM194324.CEL.gz 6.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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