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Sample GSM194343 Query DataSets for GSM194343
Status Public on Aug 09, 2008
Title Mouse Myc-induced lymphoma 114
Sample type RNA
 
Source name Lymphoma from Em-myc transgenic mouse
Organism Mus musculus
Characteristics Genotype: Em-myc transgenic
Onset of age: 265
Extracted molecule total RNA
Extraction protocol Snap-frozen samples in liquid nitrogen were used for extraction. Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 24, 2007
Last update date Aug 28, 2018
Contact name Seiichi Mori
E-mail(s) seiichi.mori@nus.edu.sg
Phone (65) 6516 1148
Fax (65) 6873 9664
URL http://www.csi.nus.edu.sg
Organization name National University of Singapore
Department Cancer Science Institute Singapore
Lab Genomic Oncology Programme
Street address Centre for Life Sciences #02-07, 28 Medical Drive
City Singapore
State/province Singapore
ZIP/Postal code 117456
Country Singapore
 
Platform ID GPL1261
Series (1)
GSE7897 Expression data from Mouse Lymphoma
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1450.083579
1415671_at 1621.765066
1415672_at 7173.461324
1415673_at 985.9903807
1415674_a_at 1367.347792
1415675_at 1061.730162
1415676_a_at 5514.000103
1415677_at 385.8502979
1415678_at 2001.902645
1415679_at 5213.103818
1415680_at 2408.860852
1415681_at 1816.30457
1415682_at 1848.332732
1415683_at 3155.195685
1415684_at 449.3079252
1415685_at 1147.134935
1415686_at 1351.525102
1415687_a_at 11311.59147
1415688_at 4365.721004
1415689_s_at 1434.056359

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM194343.CEL.gz 6.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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