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Sample GSM194345 Query DataSets for GSM194345
Status Public on Aug 09, 2008
Title Mouse Myc-induced lymphoma 117
Sample type RNA
 
Source name Lymphoma from Em-myc transgenic mouse
Organism Mus musculus
Characteristics Genotype: Em-myc transgenic
Onset of age: 319
Extracted molecule total RNA
Extraction protocol Snap-frozen samples in liquid nitrogen were used for extraction. Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 24, 2007
Last update date Aug 28, 2018
Contact name Seiichi Mori
E-mail(s) seiichi.mori@nus.edu.sg
Phone (65) 6516 1148
Fax (65) 6873 9664
URL http://www.csi.nus.edu.sg
Organization name National University of Singapore
Department Cancer Science Institute Singapore
Lab Genomic Oncology Programme
Street address Centre for Life Sciences #02-07, 28 Medical Drive
City Singapore
State/province Singapore
ZIP/Postal code 117456
Country Singapore
 
Platform ID GPL1261
Series (1)
GSE7897 Expression data from Mouse Lymphoma
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1803.701448
1415671_at 1571.657905
1415672_at 5506.066225
1415673_at 1033.519592
1415674_a_at 1260.266972
1415675_at 1198.504186
1415676_a_at 5972.077306
1415677_at 442.589593
1415678_at 1540.076059
1415679_at 5374.603726
1415680_at 1712.243677
1415681_at 1564.489459
1415682_at 1441.255973
1415683_at 3990.199221
1415684_at 1403.705578
1415685_at 1131.546574
1415686_at 2042.799135
1415687_a_at 19550.84669
1415688_at 2637.06178
1415689_s_at 1232.029338

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM194345.CEL.gz 6.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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