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Sample GSM194355 Query DataSets for GSM194355
Status Public on Aug 09, 2008
Title Mouse Myc-induced lymphoma 128
Sample type RNA
 
Source name Lymphoma from Em-myc transgenic mouse
Organism Mus musculus
Characteristics Genotype: Em-myc transgenic
Onset of age: 412
Extracted molecule total RNA
Extraction protocol Snap-frozen samples in liquid nitrogen were used for extraction. Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 24, 2007
Last update date Aug 28, 2018
Contact name Seiichi Mori
E-mail(s) seiichi.mori@nus.edu.sg
Phone (65) 6516 1148
Fax (65) 6873 9664
URL http://www.csi.nus.edu.sg
Organization name National University of Singapore
Department Cancer Science Institute Singapore
Lab Genomic Oncology Programme
Street address Centre for Life Sciences #02-07, 28 Medical Drive
City Singapore
State/province Singapore
ZIP/Postal code 117456
Country Singapore
 
Platform ID GPL1261
Series (1)
GSE7897 Expression data from Mouse Lymphoma
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1778.391989
1415671_at 1332.090139
1415672_at 4611.40175
1415673_at 871.3052203
1415674_a_at 1311.125491
1415675_at 1143.457691
1415676_a_at 5334.214477
1415677_at 683.533116
1415678_at 1542.999932
1415679_at 6259.17127
1415680_at 1982.830323
1415681_at 2251.178409
1415682_at 915.094432
1415683_at 4033.925501
1415684_at 785.9128291
1415685_at 1219.442339
1415686_at 1465.727913
1415687_a_at 17508.51077
1415688_at 2267.216991
1415689_s_at 959.3653498

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM194355.CEL.gz 6.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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