NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM194366 Query DataSets for GSM194366
Status Public on Aug 09, 2008
Title Mouse Wildtype Normal Lymph Node 2
Sample type RNA
 
Source name Mouse normal lymph node
Organism Mus musculus
Characteristics Genotype: Wildtype
Sampling age: 38
Extracted molecule total RNA
Extraction protocol Snap-frozen samples in liquid nitrogen were used for extraction. Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 24, 2007
Last update date Aug 28, 2018
Contact name Seiichi Mori
E-mail(s) seiichi.mori@nus.edu.sg
Phone (65) 6516 1148
Fax (65) 6873 9664
URL http://www.csi.nus.edu.sg
Organization name National University of Singapore
Department Cancer Science Institute Singapore
Lab Genomic Oncology Programme
Street address Centre for Life Sciences #02-07, 28 Medical Drive
City Singapore
State/province Singapore
ZIP/Postal code 117456
Country Singapore
 
Platform ID GPL1261
Series (1)
GSE7897 Expression data from Mouse Lymphoma
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1415670_at 1631.659528
1415671_at 2224.243219
1415672_at 4620.31111
1415673_at 445.7398997
1415674_a_at 1177.099276
1415675_at 1088.072018
1415676_a_at 2854.646971
1415677_at 720.0973993
1415678_at 1650.35914
1415679_at 5694.171009
1415680_at 1484.682817
1415681_at 1240.78611
1415682_at 881.2251544
1415683_at 3071.861823
1415684_at 743.5153619
1415685_at 1011.383355
1415686_at 1714.039376
1415687_a_at 23015.80277
1415688_at 2523.401032
1415689_s_at 1205.19137

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM194366.CEL.gz 6.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap