|
| Status |
Public on Aug 09, 2008 |
| Title |
Mouse Wildtype Normal Lymph Node 5 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse normal lymph node
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: Wildtype
Sampling age: 52
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Snap-frozen samples in liquid nitrogen were used for extraction. Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from embryos younger than nuclear cycle 9, i.e. before zygotic genome activation.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Seiichi Mori |
| E-mail(s) |
seiichi.mori@nus.edu.sg
|
| Phone |
(65) 6516 1148
|
| Fax |
(65) 6873 9664
|
| URL |
http://www.csi.nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute Singapore
|
| Lab |
Genomic Oncology Programme
|
| Street address |
Centre for Life Sciences #02-07, 28 Medical Drive
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7897 |
Expression data from Mouse Lymphoma |
|
| Relations |
| Reanalyzed by |
GSE119085 |
