|
| Status |
Public on May 26, 2007 |
| Title |
GT1 |
| Sample type |
RNA |
| |
|
| Source name |
genital tubercle
|
| Organism |
Mus musculus |
| Characteristics |
gene reported: SMAA/EYFP transgenic
strain: FVB/N
Sex: male
developmental stage: E14
theiler stage: 22
somite count: NA
developmental landmark: Distal borders of anterior and posterior footplates indented; digit widths and locations discernable.
|
| Treatment protocol |
pool size: N/A
Pooled sample: no
Dissection Method: Genital tubercle region micro-dissected based on SMAA/EYFP expression.
Female FVB/N mice were time-mated to SMAA/EYFP transgenic males. Pregnant FVB/N mice were euthanized by standard carbon dioxide asphyxiation at 14 days post-coitus. Embryonic samples were euthanized by decapitation and tissues were micro-dissected, rapidly frozen and stored at -80ºC until RNA was extracted for gene expression analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen: RNeasy
|
| Label |
Biotin
|
| Label protocol |
Biotin-X-X-NHS (EPICENTRE Biotechnologies)
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
Amount labeled target hybridization to array: 10 ug
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
>> Amplification protocol <<
Target Amplified manufacturer/kit: TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 (EPICENTRE Biotechnologies)
Target Amplified protocol: Potter protocols
Rounds of amplification: 2
URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:7981
|
| Data processing |
Analysis method: Affymetrix GCOS and Gene Spring and Avadis programs GCOS Tgt value: 1500
|
| |
|
| Submission date |
May 25, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
gudmap-db@gudmap.org
|
| Phone |
+44 131 651 8500
|
| Organization name |
IGMM MRC Human Genetics Unit
|
| Lab |
GUDMAP Database Group
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7903 |
Gene expression analysis of male E14 genital tubercle isolated from SMAA (Acta2) transgenic mice. (GUDMAP Series ID: 16) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
